PNR-3-80
Based on 1 Customer Validation
PNR-3-80 is an endonuclease G (ENDOG) inhibitor with an IC50 of 0.67 μM. As a non-competitive binder to the ENDOG-substrate complex, PNR-3-80 specifically inhibits the endonuclease activity of ENDOG. PNR-3-80 reduces cell death induced by Cisplatin (HY-17394) and Docetaxel (HY-B0011), and inhibits DNA damage and autophagy (autophagy) induced by Etoposide (HY-13629). PNR-3-80 can be used in studies related to cell injury.
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- CAS. Nr.: 1424353-63-8
- Formel: C24H14ClN3O3S
- Molecular Weight:459.90
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Anti-Cancer Compound Library
HY-L025
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Autophagy Compound Library
HY-L029
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Anti-Aging Compound Library
HY-L034
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Highly Selective Inhibitors Library
HY-L158
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Anti-Brain Cancer Compound Library
HY-L188
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High-Throughput Bioactive Compound Library
HY-L205
Biologische Aktivität
PNR-3-80 (50 μM; 24 h) inhibits endonuclease G (ENDOG)-dependent DNA damage and autophagy induction in human hepatocytes[1].
PNR-3-80 potently inhibits recombinant mouse EndoG in high-throughput luciferase reporter gene assays, with an IC50 of 0.67 μM[2].
PNR-3-80 (1 μM for 1 h against mouse EndoG; 1 mM for 1 h against human EndoG) protects plasmid DNA from degradation by EndoG in plasmid nicking assays[2].
PNR-3-80 (pre-incubated for 2 h followed by co-incubation with Cisplatin for 24 h at a concentration of 50 μM) significantly protects 22Rv1 human prostate cancer cells that endogenously express EndoG against Cisplatin-induced cell death[2].
PNR-3-80 (preincubated for 2 h followed by co-incubation with docetaxel for 24 h at a concentration of 50 μM) significantly protects human prostate adenocarcinoma PC3 cells overexpressing EndoG from Docetaxel (HY-B0011)-induced cell death and DNA fragmentation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human prostate carcinoma 22Rv1 cells
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Concentration:50 μM
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Incubation Time:2 h pre-incubation, followed by 24 h co-incubation with Cisplatin
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Result:Significantly inhibited Cisplatin-induced LDH release, reducing Cisplatin-induced cell death compared to controls without inhibitor.
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Cell Line:EndoG-overexpressing human prostate adenocarcinoma PC3 cells
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Concentration:50 μM
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Incubation Time:2 h pre-incubation, followed by 24 h co-incubation with Docetaxel
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Result:Significantly inhibited Docetaxel-induced LDH release, reducing Docetaxel-induced cell death compared to controls without inhibitor.
Significantly decreased Docetaxel-induced DNA fragmentation as measured by TUNEL staining.
Chemical Information
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CAS. Nr. 1424353-63-8
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Appearance Solid
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Molecular Weight 459.90
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Formel C24H14ClN3O3S
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Color Light yellow to yellow
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SMILES
O=C1NC(NC(/C1=C\C2=CN(C3=C2C=C(Cl)C=C3)C(C4=CC5=C(C=CC=C5)C=C4)=O)=O)=S
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Reinheit & Dokumentation
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Data Sheet (279 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Wang W, et al. Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. Nat Commun. 2021;12(1):476. Published 2021 Jan 20. [Content Brief]
[2]. Jang DS, et al. Novel cytoprotective inhibitors for apoptotic endonuclease G. DNA Cell Biol. 2015;34(2):92-100. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
PNR-3-80 Related Classifications
HY-176835 Related Classifications
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