ACES
Based on 1 Customer Validation
ACES (N-(2-Acetamido)-2-aminoethanesulfonic acid) is a zwitterionic buffer. The working pH range of ACES buffer is 6.8-7.2.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 7365-82-4
- Formula: C4H10N2O4S
- Molecular Weight:182.20
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
Biological Activity
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 7365-82-4
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Appearance Solid
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Molecular Weight 182.20
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Formula C4H10N2O4S
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Color White to off-white
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SMILES
O=C(N)CNCCS(=O)(O)=O
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Synonyms
N-(2-Acetamido)-2-aminoethanesulfonic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
H2O : 40 mg/mL (219.54 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 16.67 mg/mL (91.49 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (269 KB)
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SDS (477 KB)
- English - EN (477 KB)
- Français - FR (477 KB)
- Deutsch - DE (477 KB)
- Norwegian - NO (477 KB)
- Español - ES (477 KB)
- Swedish - SV (477 KB)
- Italian - IT (477 KB)
- Korean - KR (477 KB)
- Portuguese - PT (477 KB)
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Handling Instructions (2659 KB)
References
[1]. Melissa D Mertzman, et al. Effect of surfactant concentration and buffer selection on chromatographic figures of merit in chiral microemulsion electrokinetic chromatography. Electrophoresis. 2004 Oct;25(18-19):3247-56. [Content Brief]
[2]. Rabindra N Roy, et al. Buffer Standards for the Physiological pH of the Zwitterionic Compound, ACES from 5 to 55°C. J Solution Chem. 2009 Apr 1;38(4):471-483. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 5.4885 mL | 27.4424 mL | 54.8847 mL | 137.2119 mL |
| 5 mM | 1.0977 mL | 5.4885 mL | 10.9769 mL | 27.4424 mL | |
| 10 mM | 0.5488 mL | 2.7442 mL | 5.4885 mL | 13.7212 mL | |
| 15 mM | 0.3659 mL | 1.8295 mL | 3.6590 mL | 9.1475 mL | |
| 20 mM | 0.2744 mL | 1.3721 mL | 2.7442 mL | 6.8606 mL | |
| 25 mM | 0.2195 mL | 1.0977 mL | 2.1954 mL | 5.4885 mL | |
| 30 mM | 0.1829 mL | 0.9147 mL | 1.8295 mL | 4.5737 mL | |
| 40 mM | 0.1372 mL | 0.6861 mL | 1.3721 mL | 3.4303 mL | |
| 50 mM | 0.1098 mL | 0.5488 mL | 1.0977 mL | 2.7442 mL | |
| 60 mM | 0.0915 mL | 0.4574 mL | 0.9147 mL | 2.2869 mL | |
| 80 mM | 0.0686 mL | 0.3430 mL | 0.6861 mL | 1.7151 mL | |
| 100 mM | 0.0549 mL | 0.2744 mL | 0.5488 mL | 1.3721 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.