RhFNMB

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of RhFNMB working solution
1.1 Prepare a 10 mM stock solution
1.2 Prepare a 5-10 μM RhFNMB working solution
Note: Please adjust the concentration of RhFNMB working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 min and then discard the supernatant. Wash twice with PBS, 5 min each time. The cell density is 1×10⁶/mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 1 h.
2.3 Centrifuge at 400 g at 4°C for 3-4 min and then discard the supernatant.
2.4 Wash twice with PBS, 5 min each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 1 h.
3.4 Wash twice with medium, 5 min each time. Observation by fluorescence microscopy or flow cytometry.
Precautions
1. Please adjust the concentration of RhFNMB working solution according to the actual situation.
2. This product is only for R&D use, not for drug, household, or others.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.