AMC-GlcNAc
AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate.
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- Formule: C19H22N2O9
- Masse moléculaire:422.39
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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- Pureté et documentation
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Help & FAQs
Activité biologique
Description
Emission (Em)
480
Excitation (Ex)
390
Chemical Information
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Masse moléculaire 422.39
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Formule C19H22N2O9
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SMILES
CC(C(C1=C2)=CC=C2NC(O[C@H]3[C@H](NC(C)=O)[C@@H](O)[C@H](O)[C@@H](CO)O3)=O)=CC(O1)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Detergent-Based Total Protein Extraction from Cultured Mammalian Cells (RIPA/NP-40/Triton Lysis)
Detergent-based total protein extraction lyses cultured mammalian cells by using amphipathic detergents to disrupt lipid membranes and solubilize proteins into aqueous lysate; RIPA combines non-ionic detergent with ionic detergents, whereas NP-40/Triton buffers are milder non-ionic lysis conditions. The readout is the recovered clarified protein lysate, which is commonly quantified by detergent-compatible protein assays and then used for downstream protein analysis such as SDS-PAGE and Western blotting.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AMC-GlcNAc Related Classifications
HY-143285 Related Classifications
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