DiOC3(3)
DiOC3(3) (3,3'-Dipropyloxacarbocyanine iodide) is a green fluorescent lipophilic dye with cell membrane permeability. DiOC3(3) can be used to stain cell membranes and other lipid-soluble biological structures.
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- CAS No.: 53213-79-9
- Formule: C23H25IN2O2
- Masse moléculaire:488.36
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
DiOC3(3) is effluxed to a marked degree relative to the DiOC5-7(3) in the KB85 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Emission (Em)
525
Excitation (Ex)
495
Chemical Information
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CAS No. 53213-79-9
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Masse moléculaire 488.36
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Formule C23H25IN2O2
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SMILES
CCCN1/C(OC2=CC=CC=C12)=C\C=C\C3=[N+](CCC)C4=CC=CC=C4O3.[I-]
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Synonyms
3,3'-Dipropyloxacarbocyanine iodide
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Transepithelial/transendothelial electrical resistance assay
TEER measures electrical resistance across epithelial or endothelial monolayers cultured on permeable supports, and the readout reflects ionic conductance through the cell barrier, especially the paracellular pathway regulated by junctional integrity. TEER can be measured without destroying the monolayer and is commonly used before or during transport, permeability, barrier-disruption, and barrier-maturation experiments. TEER values are influenced by biological maturation and technical conditions; reported factors include temperature, medium formulation, passage number, electrode geometry, membrane properties, and junctional length during early monolayer maturation. Therefore, TEER should be interpreted with blank-insert subtraction, area normalization, repeated readings, and, when possible, orthogonal barrier readouts such as FITC-dextran flux or tight-junction staining.
Pureté et documentation
Références
[1]. Wadkins RM, et al. Kinetics of transport of dialkyloxacarbocyanines in multidrug-resistant cell lines overexpressing P-glycoprotein: interrelationship of dye alkyl chain length, cellular flux, and drug resistance. Biochemistry. 1995 Mar 21;34(11):3858-72. [Content Brief]
[2]. Kawabe Y, et al. Effects of DNA on the optical properties of cyanine dyes. Proc SPIE. 2010.
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DiOC3(3) Related Classifications
HY-D1074 Related Classifications
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