PDK-IN-3
Based on 1 Customer Validation
PDK-IN-3 (compound 1) is a potent pan inhibitor of PDK with IC50s of 109.3, 135.8, 458.7 nM and 8.67 μM against PDK 1-4, respectively. PDK-IN-3 also induces proliferation and apoptosis in A549 cells.
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- Pureté: 99.87%
- CAS No.: 3057973-22-2
- Formule: C16H19ClN2O5S2
- Masse moléculaire:418.92
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | EC50 |
10.7 μM
Compound: 1
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Anticancer activity against human A549 cells assessed as inhibition of cell growth incubated for 24 to 72 hrs by IncuCyte Zoom live cell imaging analysis
Anticancer activity against human A549 cells assessed as inhibition of cell growth incubated for 24 to 72 hrs by IncuCyte Zoom live cell imaging analysis
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[PMID: 38056298] |
| A549 | IC50 |
5.67 μM
Compound: 1
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Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs under hypoxic condition by MTT assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs under hypoxic condition by MTT assay
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[PMID: 38056298] |
Chemical Information
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CAS No. 3057973-22-2
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Appearance Solid
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Masse moléculaire 418.92
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Formule C16H19ClN2O5S2
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Color White to off-white
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SMILES
CC1=CC(C)=C(C=C1S(=O)(NCCO)=O)NS(=O)(C2=CC=C(C=C2)Cl)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 66.67 mg/mL (159.15 mM; ultrasonic and warming and adjust pH to 2 with 1 M HCl and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3871 mL | 11.9355 mL | 23.8709 mL | 59.6773 mL |
| 5 mM | 0.4774 mL | 2.3871 mL | 4.7742 mL | 11.9355 mL | |
| 10 mM | 0.2387 mL | 1.1935 mL | 2.3871 mL | 5.9677 mL | |
| 15 mM | 0.1591 mL | 0.7957 mL | 1.5914 mL | 3.9785 mL | |
| 20 mM | 0.1194 mL | 0.5968 mL | 1.1935 mL | 2.9839 mL | |
| 25 mM | 0.0955 mL | 0.4774 mL | 0.9548 mL | 2.3871 mL | |
| 30 mM | 0.0796 mL | 0.3978 mL | 0.7957 mL | 1.9892 mL | |
| 40 mM | 0.0597 mL | 0.2984 mL | 0.5968 mL | 1.4919 mL | |
| 50 mM | 0.0477 mL | 0.2387 mL | 0.4774 mL | 1.1935 mL | |
| 60 mM | 0.0398 mL | 0.1989 mL | 0.3978 mL | 0.9946 mL | |
| 80 mM | 0.0298 mL | 0.1492 mL | 0.2984 mL | 0.7460 mL | |
| 100 mM | 0.0239 mL | 0.1194 mL | 0.2387 mL | 0.5968 mL |
PDK-IN-3 Related Classifications
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