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Cat. No.: HY-P12209

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CKRPRAASFAE

CKRPRAASFAE is a glycogen synthase kinase 3-β-derived PKB/Akt peptide substrate that is used for in vitro kinase activity assays.

For research use only. We do not sell to patients.

CAS No.: 312773-04-9
Formula: C₅₂H₈₆N₁₈O₁₅S
Molecular Weight:1235.42

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Custom Peptide Synthesis

CKRPRAASFAE Chemical Structure

CAS No.: 312773-04-9

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

CKRPRAASFAE is a glycogen synthase kinase 3-β-derived PKB/Akt peptide substrate that is used for in vitro kinase activity assays^[1]^[2]^[3].

IC₅₀ & Target

^[1]^[2]

Akt

SGK3

All Akt Isoforms :

All SGK Isoforms :

In Vitro

CKRPRAASFAE phosphorylates doubly phosphorylated ppAkt1^T308,S473 with the highest efficiency, followed by singly phosphorylated pAkt1^T308, pAkt1^S473 and unphosphorylated Akt1, whereas phospho-mimetic and mutant Akt1 variants exhibit extremely low activity towards this peptide^[1].
CKRPRAASFAE (5 μg; 15 min) acts as a substrate for SGK3 immunoprecipitated from 293T cells^[2].
CKRPRAASFAE TAMRA fluorescent derivative acts as a specific substrate for purified Akt1 kinase, and enables quantitative detection of Akt1 activity via TMSP‑based dual‑readout fluorometric assay^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 312773-04-9
Molecular Weight 1235.42
Formula C₅₂H₈₆N₁₈O₁₅S
Sequence

Cys-Lys-Arg-Pro-Arg-Ala-Ala-Ser-Phe-Ala-Glu
Sequence Shortening

CKRPRAASFAE
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Balasuriya N, et al. Genetic code expansion and live cell imaging reveal that Thr-308 phosphorylation is irreplaceable and sufficient for Akt1 activity. The Journal of biological chemistry. 2018 Jul 06;293(27):10744-10756.

[2]. Wang Y, et al. Coordinated regulation of serum- and glucocorticoid-inducible kinase 3 by a C-terminal hydrophobic motif and Hsp90-Cdc37 chaperone complex. The Journal of biological chemistry. 2014 Feb 21;289(8):4815-26.

[3]. Bai J, et al. Dual-readout fluorescent assay of protein kinase activity by use of TiO2-coated magnetic microspheres. Analytical chemistry. 2013 May 07;85(9):4813-21.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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CKRPRAASFAE

All Akt Isoforms

All SGK Isoforms

Biological Activity

Description

IC50 & Target

In Vitro

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

CKRPRAASFAE Related Classifications

HY-P12209 Related Classifications

Bioz

Keywords

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