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Cat. No.: HY-E70005L

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Collagenase (Type B, animal free)

Name: Collagenase (Type B, animal free)
Brand: MedChemExpress
SKU: HY-E70005L
Price: 55.00 USD
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Collagenase (Type B, animal free) is an animal-free collagen-degrading digestive enzyme and tissue dissociation reagent. Collagenase (Type B, animal free) can digest mouse aorta, myocardium, human umbilical cord, and human liver tissue to isolate primary leukocytes, adult mouse cardiomyocytes, human mesenchymal stem cells, and individual hepatocytes. Collagenase (Type B, animal free) can be used in studies related to liver cirrhosis.

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CAS No.: 9001-12-1

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Please store the product under the recommended conditions in the Certificate of Analysis.

Collagenase (Type B, animal free) Chemical Structure

CAS No.: 9001-12-1

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Activité biologique
- Description
- In Vitro
Chemical Information
Publications (1)
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Description

In Vitro

Collagenase (Type B, animal free) (1 mg/mL; 12 h) isolates human umbilical cord MSCs that are expandable in serum-based medium^[1].
Collagenase (Type B, animal free) efficiently isolates human umbilical cord MSCs that retain expandability, MSC surface marker expression, and trilineage differentiation potential in NBVbe medium^[1].
Collagenase (Type B, animal free) (2 mg/mL) is the optimal concentration for isolating human umbilical cord MSCs that are expandable in NBVbe medium, yielding the highest cell number by 30 days post-isolation^[1].
Collagenase (Type B, animal free) (2.93 mg/mL; 30 min) efficiently dissociates fresh non-ischaemic human liver tissue into a single-cell suspension suitable for scRNA-seq and flow cytometry^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

Chemical Information

CAS No. 9001-12-1
Appearance Solid
Color Light yellow to brown
SMILES

[Collagenase (Type B, animal free)]
Livraison

Room temperature in continental US; may vary elsewhere.
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Please store the product under the recommended conditions in the Certificate of Analysis.

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Naunyn Schmiedebergs Arch Pharmacol

2026 Aug 6. PMID: 42560551

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H₂O : 100 mg/mL (Need ultrasonic)

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Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (272 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Xu J, et al. Chemical-defined medium supporting the expansion of human mesenchymal stem cells. Stem cell research & therapy. 2020 Mar 19;11(1):125.

[2]. Ramachandran P, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019 Nov;575(7783):512-518.

[3]. Lai B, et al. Cost-effective storage solution for delivering umbilical cord with efficient isolation of mesenchymal stem cells. BioTechniques. 2020 Jul;69(1):410-413.

[4]. Pinz I, et al. An improved isolation procedure for adult mouse cardiomyocytes[J]. Cell biochemistry and biophysics, 2011, 61(1): 93-101.

[5]. Wan W, et al. Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice. Cardiovascular research. 2013 Mar 01;97(3):580-8.

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Collagenase (Type B, animal free)

1 Cited Publications

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Activité biologique
- Description
- In Vitro
Chemical Information
Publications (1)
Solvant et solubilité
- In Vitro
Pureté et documentation
Références

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Collagenase (Type B, animal free)

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