Collagenase (Type C, animal free)
Based on 1 Customer Validation
Collagenase (Type C, animal free) is a proteolytic enzyme targeting collagen, capable of releasing corneal endothelial cells (CECs) without damaging cell junctions.
For research use only. We do not sell to patients.
- CAS No.: 9001-12-1
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-E70005D
Collagenase IV, Clostridium histolyticaZinc peptidase, Matrix metalloproteinases (MMPs) -
HY-E70005A
Collagenase, Type IZinc peptidase, Matrix metalloproteinases (MMPs) -
HY-E70005B
Collagenase, Type IIZinc peptidase, Matrix metalloproteinases (MMPs) -
HY-E70005H
Collagenase, Type VIIIProtease -
HY-O0004
Collagenase I, from Clostridium histolyticum -
HY-E70005N
Collagenase (Type D, animal free)Collagenase -
HY-E70005J
Collagenase, Type VIICollagenase -
HY-E70005C
Collagenase, Type IIIZinc peptidase, Matrix metalloproteinases (MMPs) -
HY-E70005I
Collagenase, Type VICollagenase -
HY-E70005K
Collagenase (Type A, animal free)Collagenase -
HY-E70005E
Collagenase, Type VZinc peptidase, Matrix metalloproteinases (MMPs) -
HY-E70005L
Collagenase (Type B, animal free)Tissue-Dissolving Agent
Biological Activity
Description
In Vitro
Collagenase (Type C, animal-free) can be used to isolate and prepare single-cell suspensions from various tissues for establishing primary cell culture systems. The suitability of each batch of enzyme for dissociating specific tissues must be verified experimentally[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 9001-12-1
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Appearance Solid
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Color Light yellow to brown
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SMILES
[Collagenase (Type C, animal free)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
H2O : 50 mg/mL (Need ultrasonic)
Protocols
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Endothelial Tube Formation Assay
Endothelial tube formation assay evaluates the ability of endothelial cells to attach, migrate, align, and organize into capillary-like networks when cultured on gelled basement membrane extract or Matrigel; the readout is the morphology and quantity of tube-like networks, which reflects an in vitro endothelial morphogenesis step related to angiogenesis. Basement membrane extract/Matrigel provides laminin-rich extracellular matrix cues that support endothelial differentiation into capillary-like structures, but it can contain biologically active growth factors, so growth-factor-reduced matrix is preferred when testing defined angiogenic stimulators or inhibitors.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Collagenase (Type C, animal free) Related Classifications
HY-E70005M Related Classifications
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