2. Apoptosis
  3. Apoptosis Caspase
  4. Copper(II) diethyldithiocarbamate

Copper(II) diethyldithiocarbamate  (Synonyms: Diethyldithiocarbamic acid copper salt)

Cat. No.: HY-W111581 Purity: 98.5%
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Handling Instructions Technical Support

Copper(II) diethyldithiocarbamate (Diethyldithiocarbamic acid copper salt) is a Copper(II) and diethyldithiocarbamate coordination polymer. Copper(II) diethyldithiocarbamate binds tightly to NPL4 and induces its aggregation, disrupting the p97-NPL4-UFD1 pathway. Copper(II) diethyldithiocarbamate causes ubiquitinated proteins accumulation and impairs waste proteins degradation, thus resulting in cell apoptosis. Copper(II) diethyldithiocarbamate inhibits tumor growth in MDA-MB-231 xenograft mice models. Copper(II) diethyldithiocarbamate can be used for the research of cancer, such as breast cancer.

For research use only. We do not sell to patients.

Copper(II) diethyldithiocarbamate

Copper(II) diethyldithiocarbamate Chemical Structure

CAS No. : 13681-87-3

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Based on 1 publication(s) in Google Scholar

Copper(II) diethyldithiocarbamate Related Antibodies

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1 Publications Citing Use of MCE Copper(II) diethyldithiocarbamate

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Description

Copper(II) diethyldithiocarbamate (Diethyldithiocarbamic acid copper salt) is a Copper(II) and diethyldithiocarbamate coordination polymer. Copper(II) diethyldithiocarbamate binds tightly to NPL4 and induces its aggregation, disrupting the p97-NPL4-UFD1 pathway. Copper(II) diethyldithiocarbamate causes ubiquitinated proteins accumulation and impairs waste proteins degradation, thus resulting in cell apoptosis. Copper(II) diethyldithiocarbamate inhibits tumor growth in MDA-MB-231 xenograft mice models. Copper(II) diethyldithiocarbamate can be used for the research of cancer, such as breast cancer[1][2].

IC50 & Target[1]

Caspase 3

 

In Vitro

Copper(II) diethyldithiocarbamate (Compound CuET) (16-4000 nM; 48 h) delivered via CuET NPs and CuET@HA NPs is more cytotoxic to MDA-MB-231 breast cancer cells (IC50 115 nM and 97 nM, respectively) than to normal HEK-293 kidney cells (IC50 389 nM and 407.4 nM, respectively)[1].
Copper(II) diethyldithiocarbamate (0.1-1.0 μM; 24 h) delivered via CuET NPs induces concentration-dependent accumulation of poly-ubiquitylated proteins in MDA-MB-231 cells, confirming inhibition of the p97-NPL4 pathway[1].
Copper(II) diethyldithiocarbamate (0.5-5 μM; 24 h) delivered via CuET NPs and CuET@HA NPs induces concentration-dependent cell death in MDA-MB-231 cells after 24 h of incubation[1].
Copper(II) diethyldithiocarbamate (100-200 nM; 48 h) delivered via CuET NPs and CuET@HA NPs significantly inhibits colony formation in MDA-MB-231 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Copper(II) diethyldithiocarbamate Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: MDA-MB-231 (triple negative breast cancer) cells, HEK-293 (human embryo kidney) cells
Concentration: 16, 32, 64, 125, 250, 500, 1000, 2000, 4000 nM (equivalent diethyldithiocarbamate concentration)
Incubation Time: 48 h
Result: Showed concentration-dependent cytotoxicity in MDA-MB-231 cells, with CuET@HA NPs exhibiting higher activity at each concentration.
Reached an IC50 of 115 nM in MDA-MB-231 cells (CuET NPs).
Reached an IC50 of 97 nM in MDA-MB-231 cells (CuET@HA NPs).
Reached an IC50 of 389 nM in HEK-293 cells (CuET NPs).
Reached an IC50 of 407.4 nM in HEK-293 cells (CuET@HA NPs).
In Vivo

Copper(II) diethyldithiocarbamate (Compound CuET) (1 mg/kg; i.v.) delivered via CuET NPs and CuET@HA NPs targets MDA-MB-231 xenograft tumors and shows tumor inhibitory activity in BALB/c nude mice, while maintaining low systemic toxicity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude with MDA-MB-231 xenograft (female, 6 weeks old)[1]
Dosage: 1 mg/kg (equivalent diethyldithiocarbamate dose)
Administration: i.v.
Result: Inhibited tumor growth significantly over 14 days, with CuET@HA NPs showing the most potent efficacy (slowest tumor growth rate and smallest final tumor size).
Induced the most distinct nuclear necrosis in tumor tissue on H&E staining (CuET@HA NP-treated mice).
Increased caspase-3 activation to the highest level on immunofluorescence staining (CuET@HA NP-treated mice).
Caused the greatest extent of apoptosis on TUNEL staining (CuET@HA NP-treated mice).
Caused no noticeable mouse weight loss or pathological changes in major organs (heart, liver, spleen, lung, kidney) during treatment.
Molecular Weight

360.09

Formula

C10H20CuN2S4
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

CCN(CC)C([S-]1)=[S][Cu+2]21[S-]C(N(CC)CC)=[S]2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 2 mg/mL (5.55 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7771 mL 13.8854 mL 27.7708 mL
5 mM 0.5554 mL 2.7771 mL 5.5542 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.5%

SDS (393 KB)

COA (253 KB) HNMR (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7771 mL 13.8854 mL 27.7708 mL 69.4271 mL
5 mM 0.5554 mL 2.7771 mL 5.5542 mL 13.8854 mL
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Copper(II) diethyldithiocarbamate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Copper(II) diethyldithiocarbamate13681-87-3Diethyldithiocarbamic acid copperApoptosisCaspaseubiquitinated proteinsNPL4ultraviolet irradiationapoptosistumorMDA-MB-231 breast cancer cellsbreast cancerp97-NPL4-UFD1 pathwayBALB/c nude miceHEK-293 kidney cellsInhibitorinhibitorinhibit

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