Cys(Npys)-(Arg)9 acetate
Based on 1 Customer Validation
Cys(Npys)-(Arg)9 acetate is a synthetic cationic cell-penetrating peptide with a reversible thiol-reactive nitropyridyl (Npys) group. Cys(Npys)-(Arg)9 acetate efficiently mediates the internalization and delivery of various "cargo" such as proteins and antibodies by forming reversible disulfide bonds with surface-exposed cysteine residues. Cys(Npys)-(Arg)9 acetate endows TALEN proteins with cell-penetrating activity, enabling gene knockout in mammalian cells and protein transduction in wheat microspores. Cys(Npys)-(Arg)9 acetate can be conjugated with antibodies to form cationized IgG for enhancing endosomal escape of oligonucleotides, or form siRNA delivery complexes. When the molar ratio of Cys(Npys)-(Arg)9 acetate to loaded molecules is higher than 1:1, it exerts certain cytotoxic effects on cells. Cys(Npys)-(Arg)9 acetate can be used in studies related to oral squamous cell carcinoma and HIV infection.
For research use only. We do not sell to patients.
- Purity: 95.15%
- Formula: C62H118N40O12S2·xC2H4O2
- Molecular Weight:1679.99 (free base)
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Upon conjugation with CCR5-targeting TALEN protein, Cys(Npys)-(Arg)9 acetate induces CCR5 gene knockout in HeLa cells at a frequency of approximately 16% when delivered at a peptide-to-protein ratio of 8:1 or 15:1 (1 μM; 2 hr)[1].
After conjugation of Cys(Npys)-(Arg)9 acetate to TALEN proteins targeting BMPR1A, delivery at peptide-to-protein ratios of 1:1, 2:1 or 4:1 (1 μM; 2 h) induces knockout of the BMPR1A gene in HEK 293 cells, with a maximum frequency of up to 4%[1].
Cys(Npys)-(Arg)9 acetate-conjugated TALEN proteins do not reduce the viability of HeLa cells or HEK 293 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 1679.99 (free base)
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Formula C62H118N40O12S2·xC2H4O2
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Color Off-white to light yellow
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Sequence
Cys(Nyps)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH2
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Sequence Shortening
C(Npys)RRRRRRRRR-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Liu J, et al. Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering. PLoS One. 2014;9(1):e85755. Published 2014 Jan 20. [Content Brief]
[2]. Yamayoshi A, et al. Development of Antibody-Oligonucleotide Complexes for Targeting Exosomal MicroRNA. Pharmaceutics. 2020;12(6):545. Published 2020 Jun 12. [Content Brief]
[3]. Kim SS, et al. Antibody-mediated delivery of siRNAs for anti-HIV therapy. Methods Mol Biol. 2011;721:339-353. [Content Brief]
[5]. Bilichak A, et al. Intracellular delivery of fluorescent protein into viable wheat microspores using cationic peptides. Front Plant Sci. 2015;6:666. Published 2015 Aug 28. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cys(Npys)-(Arg)9 acetate Related Classifications
HY-P4087B Related Classifications
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