Cys(Npys)-(Arg)9
Cys(Npys)-(Arg)9 is a synthetic cationic cell-penetrating peptide with a reversible thiol-reactive nitropyridyl (Npys) group. Cys(Npys)-(Arg)9 efficiently mediates the internalization and delivery of various "cargo" such as proteins and antibodies by forming reversible disulfide bonds with surface-exposed cysteine residues. Cys(Npys)-(Arg)9 endows TALEN proteins with cell-penetrating activity, enabling gene knockout in mammalian cells and protein transduction in wheat microspores. Cys(Npys)-(Arg)9 can be conjugated with antibodies to form cationized IgG for enhancing endosomal escape of oligonucleotides, or form siRNA delivery complexes. When the molar ratio of Cys(Npys)-(Arg)9 to loaded molecules is higher than 1:1, it exerts certain cytotoxic effects on cells. Cys(Npys)-(Arg)9 can be used in studies related to oral squamous cell carcinoma and HIV infection.
For research use only. We do not sell to patients.
- CAS No.: 1417221-06-7
- Formula: C62H118N40O12S2
- Molecular Weight:1679.99
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Upon conjugation with CCR5-targeting TALEN protein, Cys(Npys)-(Arg)9 induces CCR5 gene knockout in HeLa cells at a frequency of approximately 16% when delivered at a peptide-to-protein ratio of 8:1 or 15:1 (1 μM; 2 hr)[1].
After conjugation of Cys(Npys)-(Arg)9 to TALEN proteins targeting BMPR1A, delivery at peptide-to-protein ratios of 1:1, 2:1 or 4:1 (1 μM; 2 h) induces knockout of the BMPR1A gene in HEK 293 cells, with a maximum frequency of up to 4%[1].
Cys(Npys)-(Arg)9-conjugated TALEN proteins do not reduce the viability of HeLa cells or HEK 293 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa and HEK 293 cells
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Concentration:Various concentrations of conjugated TALEN protein
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Incubation Time:2 hr
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Result:Showed no apparent reduction in cell viability in either HeLa or HEK 293 cells after treatment.
Demonstrated increased toxicity only associated with decreased TALEN protein purity, not the conjugated peptide itself.
Chemical Information
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CAS No. 1417221-06-7
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Molecular Weight 1679.99
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Formula C62H118N40O12S2
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Sequence
Cys(Nyps)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH2
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Sequence Shortening
C(Npys)RRRRRRRRR-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
[1]. Liu J, et al. Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering. PLoS One. 2014;9(1):e85755. Published 2014 Jan 20. [Content Brief]
[2]. Yamayoshi A, et al. Development of Antibody-Oligonucleotide Complexes for Targeting Exosomal MicroRNA. Pharmaceutics. 2020;12(6):545. Published 2020 Jun 12. [Content Brief]
[3]. Kim SS, et al. Antibody-mediated delivery of siRNAs for anti-HIV therapy. Methods Mol Biol. 2011;721:339-353. [Content Brief]
[5]. Bilichak A, et al. Intracellular delivery of fluorescent protein into viable wheat microspores using cationic peptides. Front Plant Sci. 2015;6:666. Published 2015 Aug 28. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cys(Npys)-(Arg)9 Related Classifications
HY-P4087 Related Classifications
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