HGF Protein, Human (HEK293, His)

HGF Protein is a multifunctional glycoprotein. By binding to the transmembrane tyrosine kinase receptor Met, HGF Protein plays a key role in cell proliferation, migration, differentiation and tissue repair. Abnormalities of HGF Protein are closely associated with tumor growth, metastasis and organ fibrosis. HGF Protein, Human (HEK293, His) is a recombinant HGF protein expressed by HEK293 and tagged with C-His^[1][2][3].

HGF is a multifunctional cytokine mainly secreted by mesenchymal cells. HGF activates downstream signaling pathways by binding to the transmembrane tyrosine kinase receptor Met. HGF is indispensable for embryonic development, and knockout of related genes leads to embryonic lethality. HGF has multiple biological functions, including inducing cell proliferation, migration, morphogenesis, and angiogenesis. HGF is involved in the regeneration and repair of multiple organs such as the liver, kidney, and lung, and can improve organ damage through anti-apoptotic, anti-fibrotic, and anti-inflammatory effects. Meanwhile, abnormal activation of the HGF/Met signaling pathway is closely associated with tumor invasion and metastasis. HGF has dual functions: it can promote tissue repair and inhibit tumor progression through antagonistic mechanisms^[1][2][3].

HGF Protein (Human; 0-10 ng/mL; 72 h) promotes DNA synthesis in primary rat and human hepatocytes^[4].

HGF Protein (Human; 500 μg; intra-arterial injection; 2 times or 3 mg; intravenous injection; 5 times) induces angiogenesis in a rabbit hindlimb ischemia model, significantly promoting collateral vessel formation and improving angiographic scores^[5].

1.The ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells has an ED₅₀ value of <2 ng/mL.
2.Immobilized Human HGF, His Tag at 1 μg/mL (100 μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC₅₀ of ≤28.2 ng/mL determined by ELISA.
3. Measured by its binding ability in a functional ELISA. Immobilized Human HGF at 2 μg/mL (100 μL/well) can bind Human HGFR. The ED₅₀ for this effect is 14.09-20 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Human HGF at 2 μg/mL (100 μL/well) can bind Human HGFR. The ED₅₀ for this effect is 19.58 ng/mL.
• 
  Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED₅₀ for this effect is 1.6 ng/mL, corresponding to a specific activity is 6.25×10⁵ U/mg.

Human

HEK293

C-His

P14210-1 (Q32-S728)

3082  [NCBI]

Full Length of Isoform-1 Mature Protein

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

Approximately 30-40 kDa (β subunit) & 50-65 kDa (α subunit) & 80-100 kDa (α subunit+β subunit),based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 200mM L-arginine, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
4.Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/l02-cell.html  [Content Brief]

  [2]. Cecchi F, et al. Targeting the HGF/Met signalling pathway in cancer. Eur J Cancer. 2010 May;46(7):1260-70.  [Content Brief]

  [3]. Stella MC, et al. HGF: a multifunctional growth factor controlling cell scattering. Int J Biochem Cell Biol. 1999 Dec;31(12):1357-62.  [Content Brief]

  [4]. Nakamura T, et al. The discovery of hepatocyte growth factor (HGF) and its significance for cell biology, life sciences and clinical medicine. Proc Jpn Acad Ser B Phys Biol Sci. 2010;86(6):588-610.  [Content Brief]

  [5]. Strain AJ, et al. Native and recombinant human hepatocyte growth factors are highly potent promoters of DNA synthesis in both human and rat hepatocytes. J Clin Invest. 1991 May;87(5):1853-7.  [Content Brief]

  [6]. Morishita R, et al. Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as cytokine supplement therapy. Hypertension. 1999 Jun;33(6):1379-84.  [Content Brief]