2. Apoptosis
  3. Ferroptosis
  4. DL-Buthionine-(S,R)-sulfoximine hydrochloride

DL-Buthionine-(S,R)-sulfoximine hydrochloride  (Synonyms: Buthionine sulfoximine hydrochloride; BSO hydrochloride)

Cat. No.: HY-106376B Purity: 99.09%
COA
SDS
Handling Instructions Technical Support

DL-Buthionine-(S,R)-sulfoximine hydrochloride (Buthionine sulfoximine hydrochloride) is a potent inhibitor of glutamylcysteine synthetase biosynthesis.

For research use only. We do not sell to patients.

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
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10 mM * 1 mL in Water In-stock
Solid
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50 mg In-stock
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Customer Review

Based on 35 publication(s) in Google Scholar

Other Forms of DL-Buthionine-(S,R)-sulfoximine hydrochloride:

DL-Buthionine-(S,R)-sulfoximine hydrochloride Related Antibodies

Top Publications Citing Use of Products

35 Publications Citing Use of MCE DL-Buthionine-(S,R)-sulfoximine hydrochloride

Cell Proliferation/Viability Assay
WB
Bio/Physico-chemical Assay

    DL-Buthionine-(S,R)-sulfoximine hydrochloride purchased from MedChemExpress. Usage Cited in: Environ Int. 2025 Dec 30:207:110032.  [Abstract]

    DL-Buthionine-(S,R)-sulfoximine (BSO; 40 μM; 24 h). The protein expressions of ferroptosis-associated genes.

    DL-Buthionine-(S,R)-sulfoximine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 30;15(1):7522.  [Abstract]

    DL-Buthionine-(S,R)-sulfoximine (BSO; 0-10 μM; 48 h). To determine the effect of ferroptosis, HepG2 and SNU398 cells with or without SLC13A3 knockdown were treated with BSO for 48 h.

    DL-Buthionine-(S,R)-sulfoximine hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Aug;10(22):e2206798.  [Abstract]

    DL-Buthionine-(S,R)-sulfoximine (BSO; 10 μM; 24 h). Cell death measurements in Huh7 cells treated with donafenib+GSK‐J4 with or without BSO.

    DL-Buthionine-(S,R)-sulfoximine hydrochloride purchased from MedChemExpress. Usage Cited in: Small. 2021 Dec;17(50):e2103984.  [Abstract]

    DL-Buthionine-(S,R)-sulfoximine (BSO; 500 μM; 12 h). Change of the AuNPs under different pre-treatments.

    DL-Buthionine-(S,R)-sulfoximine hydrochloride purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2020 Sep 18;48(16):9109-9123.  [Abstract]

    DL-Buthionine-(S,R)-sulfoximine (BSO; 10 μM; 2 h). The glutathione (GSH) levels in A549 cells were measured using the Micro Reduced Glutathione (GSH) Assay Kit 2 h after IR (8 Gy). Cells were pretreated with BSO for 2 h before IR.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DL-Buthionine-(S,R)-sulfoximine hydrochloride (Buthionine sulfoximine hydrochloride) is a potent inhibitor of glutamylcysteine synthetase biosynthesis.

    IC50 & Target

    Glutamylcysteine synthetase[1]
    In Vitro

    Buthionine sulfoximine is an analogs of methionine sulfoximine and inhibits gamma-glutamylcysteine synthetase about 20 times more effectively than prothionine sulfoximine and at least 100 times more effectively than methionine sulfoximine[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DL-Buthionine-(S,R)-sulfoximine hydrochloride Related Antibodies
    In Vivo

    Treatment of mice bearing HT1080 and HT1080/DR4 xenografts with a continuous i.v infusion of nontoxic doses of D,L-Buthionine-(S,R)-sulfoximine (300 and 600 mg/kg/day) produce a 60% reduction of GSH plasma levels and greater than 95 % reduction in GSH tumor levels in both parental and multidrug-resistant tumors[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    258.77

    Formula

    C8H19ClN2O3S
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC(C(N)CCS(CCCC)(=N)=O)=O.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (386.44 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.8644 mL 19.3222 mL 38.6444 mL
    5 mM 0.7729 mL 3.8644 mL 7.7289 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (386.44 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.09%

    SDS (252 KB)

    COA (246 KB) HNMR (392 KB) RP-HPLC (252 KB) MS (101 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 3.8644 mL 19.3222 mL 38.6444 mL 96.6109 mL
    5 mM 0.7729 mL 3.8644 mL 7.7289 mL 19.3222 mL
    10 mM 0.3864 mL 1.9322 mL 3.8644 mL 9.6611 mL
    15 mM 0.2576 mL 1.2881 mL 2.5763 mL 6.4407 mL
    20 mM 0.1932 mL 0.9661 mL 1.9322 mL 4.8305 mL
    25 mM 0.1546 mL 0.7729 mL 1.5458 mL 3.8644 mL
    30 mM 0.1288 mL 0.6441 mL 1.2881 mL 3.2204 mL
    40 mM 0.0966 mL 0.4831 mL 0.9661 mL 2.4153 mL
    50 mM 0.0773 mL 0.3864 mL 0.7729 mL 1.9322 mL
    60 mM 0.0644 mL 0.3220 mL 0.6441 mL 1.6102 mL
    80 mM 0.0483 mL 0.2415 mL 0.4831 mL 1.2076 mL
    100 mM 0.0386 mL 0.1932 mL 0.3864 mL 0.9661 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    DL-Buthionine-(S,R)-sulfoximine hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DL-Buthionine-(S,R)-sulfoximineButhionine sulfoximineBSOFerroptosisInhibitorinhibitorinhibit

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