Art. -Nr.: HY-16160

Cysmethynil

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Cysmethynil is an Icmt inhibitor(IC₅₀ = 2.4 μM). Cysmethynil inhibites RAS membrane binding and EGF signal transduction. Cysmethynil prevents the cells in the G1 phase and induces autophagy. Cysmethynil inhibits PC3 cells proliferation, has synergistic effect with Paclitaxel (HY-B0015) and Doxorubicin (HY-15142A). Cysmethynil has anti-tumor effects and can be used for solid tumor (such as prostate cancer et al.) research.

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Reinheit: 99.07%
CAS. Nr.: 851636-83-4
Formel: C₂₅H₃₂N₂O
Molecular Weight:376.53

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Cysmethynil Chemical Structure

CAS. Nr.: 851636-83-4

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC₅₀ = 2.4 μM for Icmt

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	19.3 μM Compound: 1	Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 23514631]
IMR-90	IC₅₀	29.2 μM Compound: 1	Antiproliferative activity against human IMR90 cells after 72 hrs by MTT assay Antiproliferative activity against human IMR90 cells after 72 hrs by MTT assay	[PMID: 23514631]
MDA-MB-231	IC₅₀	21.8 μM Compound: 45292	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by colorimetric tetrazolium assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by colorimetric tetrazolium assay	[PMID: 20809634]
MDA-MB-231	IC₅₀	22.1 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 23514631]
PC-3	IC₅₀	21.3 μM Compound: 1	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 23514631]
Sf9	IC₅₀	1.5 μM Compound: 45292	Inhibition of recombinant ICMT expressed in Sf9 cells using [3H] AdoMet after 20 mins by scintillation counting Inhibition of recombinant ICMT expressed in Sf9 cells using [3H] AdoMet after 20 mins by scintillation counting	[PMID: 20809634]
Sf9	IC₅₀	2.4 μM Compound: cysmethynil	Inhibition of recombinant ICMT expressed in Sf9 cells using [3H]-AdoMet after 20 mins by scintillation counter Inhibition of recombinant ICMT expressed in Sf9 cells using [3H]-AdoMet after 20 mins by scintillation counter	[PMID: 21661760]
Sf9	IC₅₀	1.9 μM Compound: 1	Inhibition of recombinant Icmt (unknown origin) expressed in Sf9 cells assessed as transfer of methyl group from [3H]-S-adenosylmethionine to [3H]-biotin-S-farnesyl-L-cysteine after 30 mins Inhibition of recombinant Icmt (unknown origin) expressed in Sf9 cells assessed as transfer of methyl group from [3H]-S-adenosylmethionine to [3H]-biotin-S-farnesyl-L-cysteine after 30 mins	[PMID: 23514631]
Sf9	IC₅₀	2.4 μM Compound: 31	Inhibition of recombinant ICMT (unknown origin) expressed in sf9 cells using biotin S-farnesyl-L-cysteine as substrate Inhibition of recombinant ICMT (unknown origin) expressed in sf9 cells using biotin S-farnesyl-L-cysteine as substrate	[PMID: 23566315]

In Vitro

Cysmethynil (15 μM or 20 μM or 30 μM; 6 days) inhibits Icmt^+/+ cells and Icmt^-/-/ICMT cells proliferation, unaffects Icmt^-/-cells growth ^[1].
Cysmethynil (20-30 μM; 1-6 days) inhibits PC3 cells proliferation and results in a dose- and time-dependent reduction in the number of viable PC3 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:

PC3 cells^[2]
Concentration:

20-30 μM cysmethynil
Incubation Time:

1-6 days
Result:

Resulted in a dose- and time-dependent reduction in the number of viable PC3 cells.

In Vivo

Cysmethynil (100 mg/kg, 200 mg/kg; intraperitoneal injection; every 48 h; 28 day) without adverse effects on mice body weight, significantly impacts on tumor growth, causes both an accumulation of cells in the G1 phase and cell death ^[2].
Cysmethynil (20 mg/kg; intraperitoneal injection; three times a week; 2 weeks; alone or combined with Paclitaxel (HY-B0015)/ Doxorubicin (HY-15142A) ) is not toxic to mice and induces only moderate inhibition of tumor growth as single agent, the combination with Paclitaxel(HY-B0015)/ Doxorubicin(HY-15142A) results in significantly greater efficacy in inhibiting tumor growth. Cysmethynil sensitizes cervical cancer cells to chemotherapy agent in xenograft mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

6 week-old SCID mice with SiHa cells in the mice flank ^[3]
Dosage:

20 mg/kg (single; combination with Paclitaxel or Doxorubicin)
Administration:

intraperitoneal injection; three times a week; 2 weeks
Result:

Decreased in tumor size when treated with Cys alone, or Cysmethynil+Paclitaxel(HY-B0015)/Cysmethynil+ Doxorubicin(HY-15142A) .

Animal Model:

SCID mice with PC3 cells in a xenograft model^[2]
Dosage:

0.1 mg/g or 0.2 mg/g Cysmethynil
Administration:

intraperitoneal injection; every 48 h; 28 days
Result:

Decreased in tumor size when treated with both doses of cysmethynil.

Chemical Information

CAS. Nr. 851636-83-4
Appearance Solid
Molecular Weight 376.53
Formel C₂₅H₃₂N₂O
Color White to light yellow
SMILES

O=C(N)CC1=CN(CCCCCCCC)C2=C1C=C(C3=CC=CC(C)=C3)C=C2
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

J Ovarian Res

Vitamin B6 promotes the activation of primordial follicles through the PI3K/Akt signaling pathway. [Abstract]

2025 Dec 17;18(1):296. PMID: 41408298

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (265.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6558 mL

13.2792 mL

26.5583 mL

5 mM

0.5312 mL

2.6558 mL

5.3117 mL

10 mM

0.2656 mL

1.3279 mL

2.6558 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (251 KB)
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- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Winter-Vann AM, et al. A small-molecule inhibitor of isoprenylcysteine carboxyl methyltransferase with antitumor activity in cancer cells. Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4336-41. [Content Brief]

[2]. Wang M, et al. A small molecule inhibitor of isoprenylcysteine carboxymethyltransferase induces autophagic cell death in PC3 prostate cancer cells. J Biol Chem. 2008 Jul 4;283(27):18678-84. [Content Brief]

[3]. Pan Q, et al. Inhibition of isoprenylcysteine carboxylmethyltransferase sensitizes common chemotherapies in cervical cancer via Ras-dependent pathway. Biomed Pharmacother. 2018 Mar;99:169-175. [Content Brief]

[4]. Zhu C, et al. Targeting KRAS mutant cancers: from druggable therapy to drug resistance. Mol Cancer. 2022 Aug 4;21(1):159. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6558 mL	13.2792 mL	26.5583 mL	66.3958 mL
	5 mM	0.5312 mL	2.6558 mL	5.3117 mL	13.2792 mL
	10 mM	0.2656 mL	1.3279 mL	2.6558 mL	6.6396 mL
	15 mM	0.1771 mL	0.8853 mL	1.7706 mL	4.4264 mL
	20 mM	0.1328 mL	0.6640 mL	1.3279 mL	3.3198 mL
	25 mM	0.1062 mL	0.5312 mL	1.0623 mL	2.6558 mL
	30 mM	0.0885 mL	0.4426 mL	0.8853 mL	2.2132 mL
	40 mM	0.0664 mL	0.3320 mL	0.6640 mL	1.6599 mL
	50 mM	0.0531 mL	0.2656 mL	0.5312 mL	1.3279 mL
	60 mM	0.0443 mL	0.2213 mL	0.4426 mL	1.1066 mL
	80 mM	0.0332 mL	0.1660 mL	0.3320 mL	0.8299 mL
	100 mM	0.0266 mL	0.1328 mL	0.2656 mL	0.6640 mL

Keywords

Cysmethynil

1 Cited Publications

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Cysmethynil

GPCR/G Protein Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Prostate Cancer Compound Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Cysmethynil

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Cysmethynil

GPCR/G Protein Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Prostate Cancer Compound Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Cysmethynil

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Cysmethynil Related Classifications

HY-16160 Related Classifications