Dendroside D
Dendroside D is a sesquiterpenoid compound. Dendroside D binds to the RNA site of RNA-dependent RNA polymerase and the active site of SARS-CoV-2 main protease through hydrogen bonds, electrostatic interactions, and hydrophobic interactions, thereby blocking viral replication. Dendroside D stimulates the proliferation of mouse T and B lymphocytes, promotes T and B lymphocyte proliferation, and exhibits immunomodulatory activity. Dendroside D can be used for viral infection and immune-related research.
For research use only. We do not sell to patients.
- CAS No.: 503443-09-2
- Formula: C27H44O14
- Molecular Weight:592.64
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Dendroside D promotes the proliferation of T and B lymphocytes[3].
Dendroside D exhibits binding affinity to HAase with a docking score of -6.701 Kcal/mol in the molecular docking model[1].
Dendroside D (15 mg; 7 days) is a glycoside that yields aglycone 1a and glucose upon enzymatic hydrolysis with β-cellulase[2].
The sugar moiety of Dendroside D (0.04 M; 1 h) is D-glucose, as confirmed by GLC retention time matching authentic D-glucose[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 503443-09-2
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Molecular Weight 592.64
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Formula C27H44O14
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SMILES
C(O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)(=O)[C@@]2(C)[C@@]3([C@]2(CC[C@](CO[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)(O)[C@@]5([C@]3([C@H](C)CC5)[H])[H])[H])[H]
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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CFSE Dye Dilution Proliferation Assay
The CFSE (carboxyfluorescein diacetate succinimidyl ester) dye dilution proliferation assay is based on the covalent labeling of intracellular proteins by a cell-permeant fluorescent dye that becomes fluorescent upon intracellular ester cleavage and then is stably retained within cells. As labeled cells divide, the dye is partitioned equally between daughter cells, resulting in a stepwise halving of fluorescence intensity that can be quantified by flow cytometry to determine the number of cell divisions undergone by each cell population. This fluorescence dilution approach enables quantitative tracking of lymphocyte proliferation at the single-cell level over multiple rounds of division. CFSE-based proliferation analysis has been widely applied to measure antigen-driven lymphocyte expansion in vitro, where discrete fluorescence peaks correspond to successive cell divisions and allow reconstruction of proliferative history within heterogeneous populations.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Dendroside D Related Classifications
HY-N21759 Related Classifications
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Keywords
- Dendroside D
- 503443-09-2
- Endogenous Metabolite
- DNA/RNA Synthesis
- Lipinski's rule of five
- hyaluronidase inhibitor
- COVID-19
- SARS-CoV-2 main protease active site
- RNA-dependent RNA polymerase RNA site
- molecular docking model
- SARS-CoV-2
- sesquiterpene guaiane group compound
- mouse T and B lymphocyte proliferation
- HAase catalytic site
- Inhibitor
- inhibitor
- inhibit