Deoxyguanosine triphosphate trisodium,100 mM Solution,PCR Grade  (Synonyms: dGTP trisodium,100 mM Solution,PCR Grade; 2'-Deoxyguanosine-5'-triphosphate trisodium,100 mM Solution,PCR Grade)

Deoxyguanosine triphosphate (dGTP) trisodium,100 mM Solution,PCR Grade is one of the raw materials used by DNA polymerases to synthesize long DNA strands during DNA replication. Deoxyguanosine triphosphate trisodium,100 mM Solution,PCR Grade is used in polymerase chain reaction (PCR) for DNA amplification. This product is supplied in the form of an aqueous solution^[1][2].

Deoxyguanosine triphosphate trisodium, 100 mM Solution, PCR Grade can be replaced by 7-deaza dGTP (HY-157001) or dITP (HY-W784564) in PCR reactions to resolve sequencing-related secondary structure issues^[1].
Deoxyguanosine triphosphate trisodium can be catalytically cleaved by partially purified Escherichia coli enzyme extracts into equimolar amounts of deoxyguanosine and triphosphate; it has a K_m of 2.5 μM for dGTP and serves as the preferred deoxynucleoside triphosphate substrate for this enzyme^[2].
Deoxyguanosine triphosphate trisodium can be cleaved by dGTP hydrolase derived from *Escherichia coli*; the activity of this enzyme is inhibited by GDP, and its catalytic function depends on MgCl₂^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

573.13

C₁₀H₁₃N₅Na₃O₁₃P₃

93919-41-6

Liquid

Colorless to light yellow

O[C@H]([C@H](O1)COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)C[C@@H]1N(C=N2)C(NC(N)=N3)=C2C3=O

Room temperature in continental US; may vary elsewhere.

• [1]. Dierick H, et al. Incorporation of dITP or 7-deaza dGTP during PCR improves sequencing of the product. Nucleic Acids Res. 1993 Sep 11;21(18):4427-8.
  

  [2]. KORNBERG SR, et al. Enzymatic cleavage of deoxyguanosine triphosphate to deoxyguanosine and tripolyphosphate. J Biol Chem. 1958 Jul;233(1):159-62.