Desmethyl germinone

Desmethyl germinone (1-10 μM) directly binds to the ligand-binding pocket of rice D14L, which is evidenced by its concentration-dependent inhibitory effect on dYLG hydrolysis, with nearly complete inhibition achieved at 5-10 μM^[1].
Desmethyl germinone (25-250 μM) directly interacts with rice D14L and induces its conformational change, which is evidenced by the dose-dependent decrease in protein unfolding temperature observed in DSF assays^[1].
Desmethyl germinone (10 μM) enhances the direct interaction between rice D14L and rice D3 in vitro^[1].
Desmethyl germinone (10 μM) enhances the direct interaction between rice D14L and full-length rice OsSMAX1 as well as its D1M domain in vitro, but fails to enhance the weak interaction between rice D14L and the OsSMAX1-D2 domain^[1].
Desmethyl germinone (50-250 μM; during DSF ramp) specifically binds to Arabidopsis KAI2 protein, but does not bind to Arabidopsis AtD14 protein^[2].
Desmethyl germinone (0.1-1 μM; 7 days) specifically induces the interaction between Arabidopsis KAI2 and SMAX1 proteins in yeast two-hybrid assays, but does not induce the interaction between Arabidopsis AtD14 and SMXL7 proteins^[2].
Desmethyl germinone (1 h) inhibits AtD14-dependent YLG hydrolysis in Arabidopsis thaliana with an IC₅₀ of 162 nM; it also inhibits KAI2-dependent dMYLG hydrolysis in Arabidopsis thaliana with an IC₅₀ of 46.4 nM, indicating that it has a higher binding affinity for KAI2 than for AtD14^[2].
Desmethyl germinone (0.1-10 µM; solid agarose medium, liquid Hoagland solution, liquid induction medium; continuous exposure, 6-hour exposure, 2-hour exposure; 2-8 days) is a potent, D14L-dependent KAI2 ligand analog in rice, which inhibits mesocotyl elongation, induces dose-dependent upregulation of KL response marker genes DLK2B and OsKUF1, and promotes OsSMAX1 protein degradation^[1].