DiOC7(3)
DiOC7(3) (3,3'-Diheptyloxacarbocyanine iodide) is a green membrane potential probe (Ex=450-490 nm, Em=510-520 nm). DiOC7(3) can be used to quantify the vascular densities.
For research use only. We do not sell to patients.
- CAS No.: 53213-83-5
- Formula: C31H41IN2O2
- Molecular Weight:600.57
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vivo
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. The dye is administered to murine via lateral tail vein injection at a dose of 1 mg/kg/ in 50 μL volume.
2. Animals are killed 5 min after dye administration, and tumors are immediately excised and frozen at - 20°C.
3. Frozen sections 10 μm in thickness are rewarmed to room temperature, allowed to air dry.
4. Image using a microscope with 100W mercury lamp illumination and an epifluorescence condenser (excited at 430-490 nm, emitted at 510-520 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
510
Excitation (Ex)
475
Chemical Information
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CAS No. 53213-83-5
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Molecular Weight 600.57
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Formula C31H41IN2O2
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SMILES
CCCCCCCN1/C(OC2=CC=CC=C12)=C/C=C/C3=[N+](CCCCCCC)C4=CC=CC=C4O3.[I-]
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Synonyms
3,3'-Diheptyloxacarbocyanine iodide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Fluorescent plasma-membrane potential dye assay
Fluorescent plasma-membrane potential dye assays measure changes in cell membrane potential using voltage-sensitive dyes whose fluorescence changes when cells depolarize or hyperpolarize. Anionic bis-oxonol dyes such as DiBAC4(3) enter depolarized cells more readily and show increased fluorescence after intracellular binding, while hyperpolarization reduces dye accumulation and fluorescence. FMP/FLIPR membrane-potential dyes are used for faster, homogeneous microplate assays of ion-channel or receptor-mediated membrane-potential changes.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DiOC7(3) Related Classifications
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