Arsenazo III
Based on 1 Customer Validation
Arsenazo III is an azo derivative of chromotropic acid, a metal chrome dye, a chelating agent, and a cation complexing agent. Arsenazo III forms stable 1:1 complexes with Ca2+, Mg2+, Sr2+, Ba2+, K+, and Na+, and its binding affinity for Ca2+ depends on pH, alkali metal cation concentration, and buffer parameters. Arsenazo III serves as a colorimetric indicator for micromolar ionized Ca2+ in cells.
For research use only. We do not sell to patients.
- Purity: 95%
- CAS No.: 1668-00-4
- Formula: C22H18As2N4O14S2
- Molecular Weight:776.37
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Arsenazo III (0.74-193000 μM) forms stable, spectrally distinct complexes with alkaline earth metal ions (Ca2+ > Sr2+ > Ba2+) and K+ in buffered aqueous solutions, with a Ca-dye dissociation constant of 0.74 μM at pH 7.4 under low-ionic-strength conditions, and measurable changes in absorbance that enable quantitative determination of free Ca2+ concentrations[1].
Arsenazo III (30-40 μM; 20°C, pH 7.0, 7.4, or 7.6) has an apparent calcium binding affinity that depends on buffer type and concentration, decreasing with increasing buffer concentration and varying across imidazole-Cl, K-Hepes, and Tris-Cl at the same concentration and pH[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
560
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Chemical Information
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CAS No. 1668-00-4
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Appearance Solid
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Molecular Weight 776.37
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Formula C22H18As2N4O14S2
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Color Brown to black
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SMILES
O=S(C1=C(/N=N/C2=CC=CC=C2[As](O)(O)=O)C(O)=C3C(O)=C(/N=N/C4=CC=CC=C4[As](O)(O)=O)C(S(=O)(O)=O)=CC3=C1)(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 10 mg/mL (12.88 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (564 KB)
- English - EN (564 KB)
- Français - FR (564 KB)
- Deutsch - DE (564 KB)
- Norwegian - NO (564 KB)
- Español - ES (564 KB)
- Swedish - SV (564 KB)
- Italian - IT (564 KB)
- Korean - KR (564 KB)
- Portuguese - PT (564 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2880 mL | 6.4402 mL | 12.8805 mL | 32.2011 mL |
| 5 mM | 0.2576 mL | 1.2880 mL | 2.5761 mL | 6.4402 mL | |
| 10 mM | 0.1288 mL | 0.6440 mL | 1.2880 mL | 3.2201 mL |
Arsenazo III Related Classifications
HY-D0202 Related Classifications
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