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Cat. No.: HY-W1061585

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DNP Phosphoramidite

DNP Phosphoramidite is a non-nucleoside-based phosphoramidite carrying a 2,4-dinitrophenyl (DNP) hapten group, which enables multiple labeling of oligonucleotides during solid-phase synthesis. DNP Phosphoramidite binds to anti-DNP antibodies, allowing the detection of labeled oligonucleotides via enzyme-linked colorimetric techniques.

For research use only. We do not sell to patients.

CAS No.: 136949-85-4
Formula: C₂₁H₃₄N₅O₆P
Molecular Weight:483.50

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

DNP Phosphoramidite Chemical Structure

CAS No.: 136949-85-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

In Vitro

DNP Phosphoramidite (0.15 M; 5 h deprotection, 30 mins detritylation) enables efficient solid-phase labelling of oligonucleotides, with "trityl-on" synthesis preventing degradation during 5-hour 60°C concentrated ammonia deprotection, and resulting labelled oligonucleotides showing increased lipophilicity for easy HPLC purification^[1].
DNP Phosphoramidite enables DNP-labelled 24-mer oligonucleotides to exhibit optimal colorimetric dot blot detection sensitivity with 3 DNP labels, achieving limits comparable to biotin-labelled oligonucleotides regardless of spacer arm length^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 136949-85-4
Molecular Weight 483.50
Formula C₂₁H₃₄N₅O₆P
SMILES

CC(N(P(OCCC#N)OCCCCCCNC(C([N+]([O-])=O)=C1)=CC=C1[N+]([O-])=O)C(C)C)C
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Grzybowski J, et al. Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups. Nucleic acids research. 1993 Apr 25;21(8):1705-12. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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