Label Coupling

Janelia Fluor? 549, SE (JF549, SE) is a cell-permeable fluorescent dye with an NHS ester (succinimidyl ester (SE)) reactive group. NHS esters react with primary amines and are commonly used to conjugate dyes to proteins, antibodies, amine-modified oligonucleotides, and other molecules. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor? 549, SE also acts as a ligand for self-labeling tags such as HaloTag and SNAP-tag, and can be used in live-cell imaging studies (Ex/Em = 549 nm/571 nm).

NHS-5(6)Carboxyrhodamine is a dye used for fluorescence labeling applications, where accurate dye/protein ratios can be obtained under native conditions.

PYBG acts as a versatile precursor to be facilely conjugated with various fluorescent dyes through ‘Click chemistry’ and Sonogashira coupling reactions. PYBG is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm). Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

Sulfo SMCC R-phycoerythrin is a conjugate composed of the protein crosslinker SMCC (HY-42360) and R-PE (R-Phycoerythrin) (HY-D0988) that can be used to label proteins to make them carry red fluorescence. Among them, SMCC is able to engage antigen-coupled spleen cells to induce antigen-specific immune responses.

Potassium 2,3,3-trimethylindole-5-sulfonate (2,3,3-Trimethylindolenine-5-sulfonic acid potassium salt) is a sulfonated indoline compound, and also serves as the starting indole raw material for the synthesis of pH-sensitive near-infrared cyanine dye HCyC-646 and near-infrared fluorescent probe IR-793. Potassium 2,3,3-trimethylindole-5-sulfonate can be used in lung adenocarcinoma-related research.

Bio-17-ATP is a substrate that can be used for AMPylation assays.

Chol-N3 is a bioorthogonal-based chol probe. Chol-N3 can combine with super-resolution fluorescence microscopy (SRM), providing direct visualization of nanoscale lipid heterogeneity in the cell surface of resting living cells. Chol-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

Cy 3 Non-Sulfonated (Cyanine3) is a cyanine (Cy) dye, and a fluorescent label with green channel for protein and nucleic acid. Cy 3 Non-Sulfonated is a fluorescent photoproduct of Cyanine5 via photoconversion upon photoexcitation. Cy 3 Non-Sulfonated can be used to high-density single-particle tracking in a living cell without using UV illumination and cell-toxic additives (Ex=470 nm; Em=515 nm and 565 nm nm).

DCI-Br-3 is a rapid, highly sensitive, and selective probe to monitor thiols in the epileptic brain. (λ_ex=537 nm, λ_em=675 nm).DCI-Br-3 can effectively cross the blood-brain barrier (BBB).

1,4-Dichloro 6-carboxytetramethylrhodamine is a fluorescence labeling agent (Ex=541 nm, Em=568 nm).

DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid), an amine-reactive building block for developing a probe, can be recognized by anti-DNP antibodies. DNP-X acid is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

Disulfo-Cy3 NHS ester is a fluorescent dye used for plasma membrane protein labeling and detection antibody labeling. Disulfo-Cy3 NHS ester can covalently bind to amino groups on cell surface proteins or detection antibodies. Disulfo-Cy3 NHS ester lacks a low-affinity membrane anchoring site, avoiding transient binding to lipid membranes. Disulfo-Cy3 NHS ester, via a conjugated detection antibody, enables visualization and detection of on-cell immune sandwich structures targeting secreted proteins (Ex/Em = 550/570 nm).

JF626-HTL is a fluorescent dye conjugated from Janelia Fluor 635 and HaloTag ligand (HTL). JF626-HTL is used for far-red fluorescent labeling of HaloTag-tagged proteins, enabling live-cell imaging and dynamic tracking (Ex/Em = 626/638 nm).

AF594 C5 Maleimide is a fluorescent dye for site-specific labeling of cysteine-containing proteins. AF594 C5 Maleimide acts as an acceptor fluorophore and covalently labels cysteine residues, including Cys³¹⁶ of rhodopsin and a surface-exposed cysteine at position 252 of ClyA variants. The excitation/emission wavelength of AF594 C5 Maleimide is Ex/Em = 595/620 nm.

N-(NBD-Aminolauroyl)safingol is a fluorescent group.

AF647 Picolyl Azide is a far-red fluorescent probe used in bioorthogonal click chemistry. It reacts with terminal alkynes via copper-catalyzed azide-alkyne cycloaddition (CuAAC). The integrated picolyl group chelates copper, which speeds up the reaction and allows for lower copper concentrations to protect sensitive proteins (Ex/Em = 650/665 nm)[1].

AF647 C2 Maleimide is a thiol-reactive Fluorescent labeling reagent. AF647 C2 Maleimide reacts with free thiol groups on Fab′ molecules to form covalent conjugates, enabling fluorescent detection in capillary chip electrophoresis.

5-FAM BCN is a reactive fluorophore-labeled strained alkyne probe and bicyclononyne. 5-FAM BCN undergoes inverse-electron-demand Diels-Alder cycloaddition with five-membered cyclic dienes. 5-FAM BCN facilitates bioorthogonal bioconjugation reactions.

AF555 Picolyl Azide is a Fluorescent dye that enables site-specific fluorescent labeling via click chemistry. AF555 Picolyl Azide conjugates with the non-canonical amino acid N-propargyl-L-lysine introduced through ochre stop codon suppression via the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, achieving labeling of hyperpolarization-activated cyclic nucleotide-gated HCN channels; its picolyl group accelerates the CuAAC reaction rate, thus completing labeling rapidly at room temperature and minimizing copper-induced cytotoxicity. AF555 Picolyl Azide forms covalent bonds with the alkynyl groups of nucleotide analogs through its azide moiety, labeling viral genomes containing 5-ethynyl-2'-deoxyuridine for visualization via fluorescence microscopy.