(E)-N-(3-Methoxyphenyl)cinnamamide
(E)-N-(3-Methoxyphenyl) cinnamamide (Compound 5h) is a weakly selective inhibitor of MMP-2 and MMP-9, with weak inhibitory activity against hMMP-2 and hMMP-9, showing IC50 values of 1674.81 nM and 1658.63 nM, respectively, and low selectivity for MMP-1. (E)-N-(3-Methoxyphenyl) cinnamamide is applicable for cancer research.
For research use only. We do not sell to patients.
- CAS No.: 127033-74-3
- Formula: C16H15NO2
- Molecular Weight:253.30
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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MMP-2 1674.81 nM (IC50) |
MMP-9 1658.63 nM (IC50) |
(E)-N-(3-Methoxyphenyl) cinnamamide (compound 5h) (0.1-10000 nM; 30 min) inhibits recombinant hMMP-2 and hMMP-9 with IC50 values of 1674.81 nM and 1658.63 nM, respectively, and shows 3-fold higher selectivity for these MMPs than for recombinant hMMP-1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 127033-74-3
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Appearance Solid
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Molecular Weight 253.30
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Formula C16H15NO2
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SMILES
N(C(/C=C/C1=CC=CC=C1)=O)C2=CC(OC)=CC=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (394.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.87 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9479 mL | 19.7394 mL | 39.4789 mL | 98.6972 mL |
| 5 mM | 0.7896 mL | 3.9479 mL | 7.8958 mL | 19.7394 mL | |
| 10 mM | 0.3948 mL | 1.9739 mL | 3.9479 mL | 9.8697 mL | |
| 15 mM | 0.2632 mL | 1.3160 mL | 2.6319 mL | 6.5798 mL | |
| 20 mM | 0.1974 mL | 0.9870 mL | 1.9739 mL | 4.9349 mL | |
| 25 mM | 0.1579 mL | 0.7896 mL | 1.5792 mL | 3.9479 mL | |
| 30 mM | 0.1316 mL | 0.6580 mL | 1.3160 mL | 3.2899 mL | |
| 40 mM | 0.0987 mL | 0.4935 mL | 0.9870 mL | 2.4674 mL | |
| 50 mM | 0.0790 mL | 0.3948 mL | 0.7896 mL | 1.9739 mL | |
| 60 mM | 0.0658 mL | 0.3290 mL | 0.6580 mL | 1.6450 mL | |
| 80 mM | 0.0493 mL | 0.2467 mL | 0.4935 mL | 1.2337 mL | |
| 100 mM | 0.0395 mL | 0.1974 mL | 0.3948 mL | 0.9870 mL |
(E)-N-(3-Methoxyphenyl)cinnamamide Related Classifications
HY-W095774 Related Classifications
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