Autophagonizer
Autophagonizer (DK-1-49) is a small molecule autophagy inducer that results in an accumulation of autophagy-associated LC3-II and enhances levels of autophagosomes and acidic vacuoles. Autophagonizer inhibits cell viability and induces cell death in not only cancer cells but also Bax/Bak double-knockout cells with EC50 values of 3-4 μM.
For research use only. We do not sell to patients.
- CAS No.: 853136-76-2
- Formula: C28H31N5O3S2
- Molecular Weight:549.71
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | EC50 |
1.3 μM
Compound: DK-1-49
|
Induction of autophagy in human HeLa cells assessed as accumulation of acidic vacuoles after 24 hrs by MDC/Hoechst 33342 staining based fluorescence assay
Induction of autophagy in human HeLa cells assessed as accumulation of acidic vacuoles after 24 hrs by MDC/Hoechst 33342 staining based fluorescence assay
|
[PMID: 27597252] |
| HeLa | EC50 |
3.5 μM
Compound: DK-1-49
|
Induction of autophagic cell death in human HeLa cells after 24 hrs by MTT assay
Induction of autophagic cell death in human HeLa cells after 24 hrs by MTT assay
|
[PMID: 27597252] |
| HeLa | EC50 |
3.5 μM
Compound: DK-1-49
|
Induction of autophagy in human HeLa cells assessed as autophagosome formation after 24 hrs by Cyto-ID reagent/ Hoechst 33342 staining based fluorescence assay
Induction of autophagy in human HeLa cells assessed as autophagosome formation after 24 hrs by Cyto-ID reagent/ Hoechst 33342 staining based fluorescence assay
|
[PMID: 27597252] |
| MEF | EC50 |
2.1 μM
Compound: DK-1-49
|
Induction of autophagic cell death in wild-type MEF after 24 hrs by MTT assay
Induction of autophagic cell death in wild-type MEF after 24 hrs by MTT assay
|
[PMID: 27597252] |
| MEF | EC50 |
2.2 μM
Compound: DK-1-49
|
Induction of autophagic cell death in Bax/Bak double knockout MEF after 24 hrs by MTT assay
Induction of autophagic cell death in Bax/Bak double knockout MEF after 24 hrs by MTT assay
|
[PMID: 27597252] |
Chemical Information
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CAS No. 853136-76-2
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Molecular Weight 549.71
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Formula C28H31N5O3S2
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SMILES
O=C(C1=COC(CSC2=NC3=C(C4=C(CCCC4)S3)C(N2CC5=CC=CC=C5)=O)=N1)NCCN6CCCC6
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Synonyms
DK-1-49
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)