RUSKI-201
RUSKI-201 is a potent and specific Hedgehog acyltransferase (Hhat) inhibitor, with an IC50 of 0.20 μM. RUSKI-201 is able to block Hh signaling from Shh overexpressing cells and inhibits Hh palmitoylation. RUSKI-201 is potential Hhat chemical probe in cells and can used in studies of Hhat catalytic function.
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- No. CAS: 1458031-48-5
- Fòrmula: C20H27N3OS
- Peso molecular:357.51
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
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Cell Line
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Type | Value | Description | References |
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| HEK293 | IC50 |
0.2 μM
Compound: 3; RU-SKI201
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Inhibition of 3C-FLAG/His8-tagged human HHAT expressed in HEK293 cells assessed as reduction in enzyme-mediated sonic hedgehog (1 to 11 residues) palmitoylation by click-ELISA method
Inhibition of 3C-FLAG/His8-tagged human HHAT expressed in HEK293 cells assessed as reduction in enzyme-mediated sonic hedgehog (1 to 11 residues) palmitoylation by click-ELISA method
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[PMID: 30986059] |
| HEK293-A | CC50 |
>50 μM
Compound: 22; RUSKI-201
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Cytotoxicity against human SHH+ HEK293a cells assessed as cell viability measured after 72 hrs by MTS assay
Cytotoxicity against human SHH+ HEK293a cells assessed as cell viability measured after 72 hrs by MTS assay
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[PMID: 38198226] |
Chemical Information
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No. CAS 1458031-48-5
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Peso molecular 357.51
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Fòrmula C20H27N3OS
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SMILES
CCC(C)CNCC(N1CCC2=C(C=CS2)C1C3=NC(C)=CC=C3)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvente y solubilidad
DMSO : 100 mg/mL (279.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
Referencias
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7971 mL | 13.9856 mL | 27.9712 mL | 69.9281 mL |
| 5 mM | 0.5594 mL | 2.7971 mL | 5.5942 mL | 13.9856 mL | |
| 10 mM | 0.2797 mL | 1.3986 mL | 2.7971 mL | 6.9928 mL | |
| 15 mM | 0.1865 mL | 0.9324 mL | 1.8647 mL | 4.6619 mL | |
| 20 mM | 0.1399 mL | 0.6993 mL | 1.3986 mL | 3.4964 mL | |
| 25 mM | 0.1119 mL | 0.5594 mL | 1.1188 mL | 2.7971 mL | |
| 30 mM | 0.0932 mL | 0.4662 mL | 0.9324 mL | 2.3309 mL | |
| 40 mM | 0.0699 mL | 0.3496 mL | 0.6993 mL | 1.7482 mL | |
| 50 mM | 0.0559 mL | 0.2797 mL | 0.5594 mL | 1.3986 mL | |
| 60 mM | 0.0466 mL | 0.2331 mL | 0.4662 mL | 1.1655 mL | |
| 80 mM | 0.0350 mL | 0.1748 mL | 0.3496 mL | 0.8741 mL | |
| 100 mM | 0.0280 mL | 0.1399 mL | 0.2797 mL | 0.6993 mL |
RUSKI-201 Related Classifications
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