Ethyl nonanoate
Based on 1 Customer Validation
Ethyl nonanoate is an orally active medium-chain fatty acid ethyl ester and aroma compound present in Laimao baijiu, cachaca, and distilled spirits. Ethyl nonanoate is catalytically converted to azelaic acid as a nonanoic acid precursor derivative with cytotoxicity. Ethyl nonanoate is used as a food flavoring agent. Ethyl nonanoate exhibits antifungal activity.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 123-29-5
- Formula: C11H22O2
- Molecular Weight:186.29
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Ethyl nonanoate (up to 5 g/L; 24 h) inhibits the growth of Candida tropicalis ATCC20962 in a concentration-dependent manner, with strong inhibition observed at concentrations exceeding 5 g/L[1].
Ethyl nonanoate (0.2 g/L; 96 h) undergoes whole-cell biotransformation to Azelaic acid in Candida tropicalis ATCC20962 cells[1].
Ethyl nonanoate (1 g/L; 48-96 h) is rapidly cleaved in Candida tropicalis ATCC20962 cells to generate free nonanoic acid, and is efficiently converted to azelaic acid under synergistic induction with nonane[1].
Ethyl nonanoate (<0.6 g/L; 48-50 h) acts in Candida tropicalis ATCC20962 cells to enable sustained, highly efficient synthesis of high-concentration azelaic acid (final concentration up to 6.73 g/L, molar conversion up to 88%) via fed-batch biotransformation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Candida tropicalis ATCC20962
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Concentration:1, 2, 5 g/L
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Incubation Time:24 h
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Result:Decreased cell growth gradually with increasing concentrations.
Strongly inhibited cell growth at concentrations over 5 g/L.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 123-29-5
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Appearance Liquid (Density: 0.872 g/cm3)
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Molecular Weight 186.29
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Formula C11H22O2
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Color Colorless to light yellow
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SMILES
CCCCCCCCC(OCC)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (536.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.3680 mL | 26.8399 mL | 53.6797 mL | 134.1994 mL |
| 5 mM | 1.0736 mL | 5.3680 mL | 10.7359 mL | 26.8399 mL | |
| 10 mM | 0.5368 mL | 2.6840 mL | 5.3680 mL | 13.4199 mL | |
| 15 mM | 0.3579 mL | 1.7893 mL | 3.5786 mL | 8.9466 mL | |
| 20 mM | 0.2684 mL | 1.3420 mL | 2.6840 mL | 6.7100 mL | |
| 25 mM | 0.2147 mL | 1.0736 mL | 2.1472 mL | 5.3680 mL | |
| 30 mM | 0.1789 mL | 0.8947 mL | 1.7893 mL | 4.4733 mL | |
| 40 mM | 0.1342 mL | 0.6710 mL | 1.3420 mL | 3.3550 mL | |
| 50 mM | 0.1074 mL | 0.5368 mL | 1.0736 mL | 2.6840 mL | |
| 60 mM | 0.0895 mL | 0.4473 mL | 0.8947 mL | 2.2367 mL | |
| 80 mM | 0.0671 mL | 0.3355 mL | 0.6710 mL | 1.6775 mL | |
| 100 mM | 0.0537 mL | 0.2684 mL | 0.5368 mL | 1.3420 mL |
Ethyl nonanoate Related Classifications
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