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Cat. No.: HY-W353914

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4α-Hydroxycholesterol

Name: 4α-Hydroxycholesterol
Brand: MedChemExpress
SKU: HY-W353914
Price: 580.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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4α-Hydroxycholesterol is an isomer of 4β-Hydroxycholesterol (HY-124265). 4α‑Hydroxycholesterol is an endogenous plasma oxysterol that fails to activate the LXRα and LXRβ nuclear receptors. 4α-Hydroxycholesterol shows no obvious cytotoxicity in mouse oligodendrocytes and does not cause mitochondrial depolarization, lysosomal destabilization, excessive superoxide anion production, cytokine secretion, or cytoskeletal depolymerization. The production of 4α‑Hydroxycholesterol is independent of CYP3A enzymes, and it is often measured alongside 4β‑Hydroxycholesterol as a reference oxysterol for CYP3A biomarker studies.

For research use only. We do not sell to patients.

Purity : 99.64%
CAS No.: 34310-86-6
Formula: C₂₇H₄₆O₂
Molecular Weight:402.65

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

4α-Hydroxycholesterol Chemical Structure

CAS No.: 34310-86-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
C6	IC₅₀	>=160 μM Compound: 2, 4alpha-hydroxycholesterol, 4alpha-OHC	Cytostatic activity against rat C6 cells assessed as growth inhibition after 24 hrs by trypan blue-based cell counting method Cytostatic activity against rat C6 cells assessed as growth inhibition after 24 hrs by trypan blue-based cell counting method	[PMID: 24211631]
C6	IC₅₀	160 μM Compound: 2, 4alpha-hydroxycholesterol, 4alpha-OHC	Cytostatic activity against rat C6 cells assessed as growth inhibition after 24 hrs by trypan blue-based cell counting method Cytostatic activity against rat C6 cells assessed as growth inhibition after 24 hrs by trypan blue-based cell counting method	[PMID: 24211631]

In Vitro

ApoptosisLipid MetabolismMitochondrial FunctionLysosomal MetabolismOxidative StressMitochondrial Oxidative PhosphorylationCell Cycle RegulationOthers

Apoptosis

4α-Hydroxycholesterol (2.5-100 μM; 24 h) induces apoptotic nuclear morphology in 158N mouse oligodendrocytes without cytoplasmic vacuolation^[2].

Lipid Metabolism

4α-Hydroxycholesterol (4αOHC) (0.78-31.25 μM; 24 h) does not activate LXRα or LXRβ in HEK293T reporter gene assays^[2].

Mitochondrial Function

Lysosomal Metabolism

Oxidative Stress

4α-Hydroxycholesterol (25-50 μM; 24 h) slightly impairs lysosomal membrane stability in primary cultures of mouse oligodendrocytes at 50 μM, but does not affect mitochondrial potential or superoxide production^[2].

Oxidative Stress

Mitochondrial Function

Mitochondrial Oxidative Phosphorylation

Cell Cycle Regulation

4α-Hydroxycholesterol (2.5-100 μM; 24 h) only causes slight changes in cell cycle distribution in 158N mouse oligodendrocytes, does not affect mitochondrial transmembrane potential or lysosomal membrane integrity, does not significantly alter F-actin content, and does not stimulate superoxide anion and inflammatory cytokine production^[2].

Others

4α‑Hydroxycholesterol (4α‑OHC) is an oxysterol and an isomer of 4β‑hydroxycholesterol (HY-124265), and can serve as an analytical reference for quantitative detection studies of oxysterols^[1].
4α-Hydroxycholesterol (2.5-160 μM; 24 h) inhibits the growth of 158N mouse oligodendrocytes, with an IC₅₀ of approximately 20 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

158N murine oligodendrocytes
Concentration:

2.5, 5, 10, 20, 40, 80, 160 μM
Incubation Time:

24 h
Result:

Inhibited 158N murine oligodendrocyte growth with an IC₅₀ around 20 μM and only weakly increased cell death, reaching 20% after 24 h.

Cell Cycle Analysis^[2]

Cell Line:

158N murine oligodendrocytes
Concentration:

2.5, 12.5, 25, 50, 100 μM
Incubation Time:

24 h
Result:

Caused only minor cell-cycle distribution changes in 158N murine oligodendrocytes after 24 h.

Related Protocols :

Chemical Information

CAS No. 34310-86-6
Appearance Solid
Molecular Weight 402.65
Formula C₂₇H₄₆O₂
Color White to yellow
SMILES

C[C@@]12[C@]3([H])[C@](CC=C1[C@@H]([C@H](CC2)O)O)([H])[C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)CCCC(C)C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. de Graan AJ, et al. 4β-hydroxycholesterol as an endogenous CYP3A marker in cancer patients treated with taxanes. British journal of clinical pharmacology. 2015 Sep;80(3):560-8. [Content Brief]

[2]. Nury T, et al. Biological activities of the LXRα and β agonist, 4β-hydroxycholesterol, and of its isomer, 4α-hydroxycholesterol, on oligodendrocytes: Effects on cell growth and viability, oxidative and inflammatory status. Biochimie, 2013, 95(3): 518-530.

[3]. Nylén H. Studies on the oxysterols 4alpha- and 4beta-hydroxycholesterol. 2024.

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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