L162389
Based on 1 Customer Validation
L162389 is a potent antagonist of angiotensin AT1 receptor with Ki of 28 nM.
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- Pureza: 99.57%
- No. CAS: 169281-53-2
- Fòrmula: C31H38N4O4S
- Peso molecular:562.72
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
- Protocolo
- Pureza y Documentación
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Help & FAQs
Ver todos los productos específicos de isoformas Angiotensin Receptor
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Actividad biológica
Ki: 28 nM (angiotensin AT1 receptor)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COS-7 | IC50 |
1.5 nM
Compound: 4; L-162,389
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Displacement of 125I-[Sar1,Leu8] angiotensin-2 from rat type 1 angiotensin-2 receptor expressed in African green monkey COS7 cells after 24 hrs
Displacement of 125I-[Sar1,Leu8] angiotensin-2 from rat type 1 angiotensin-2 receptor expressed in African green monkey COS7 cells after 24 hrs
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[PMID: 26810314] |
| COS-7 | IC50 |
1.5 nM
Compound: 8, L-162389
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Displacement of [125I]-[Sar1,Leu8]angiotensin II from human AT1 receptor expressed in african green monkey COS7 cells
Displacement of [125I]-[Sar1,Leu8]angiotensin II from human AT1 receptor expressed in african green monkey COS7 cells
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[PMID: 25699147] |
L-162,389 stimulates phosphatidylinositol turnover, albeit only to a small percentage of the angiotensin response. L-162,389 acts as angiotensin antagonist with IC50 value of 105 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 169281-53-2
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Appearance Solid
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Peso molecular 562.72
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Fòrmula C31H38N4O4S
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Color White to off-white
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SMILES
O=C(OCCCC)NS(=O)(C1=CC=C(CCC)C=C1C2=CC=C(CN3C(CC)=NC4=C(C)C=C(C)N=C43)C=C2)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Protocolo
Monoiodinated 125I-[Sar1,Leu8]angiotensin II is prepared by the Iodo-Gen method. One day after transfection and 24 hr before the binding experiments, the transfected cells are transferred to 6-, 12-, or 24-well culture plates, with 0.15-9 × 105 cells/well, with a goal of total binding of 5-10% of the radiolabeled peptide. The cells are washed twice with buffer (25 mM Tris, 5 mM MgCl2, 140 mM NaCl, pH 7.4) before and after the binding. The binding is carried out for 24 hr at 4°C with 50 pm 125I-[Sar1,Leu8]angiotensin II and variable amounts of unlabeled nonpeptide or peptide ligands in 0.5-1 mL of a 25 mM Tris buffer containing 5 mm MgCl2, pH 7.4. The binding data are analyzed by computerized nonlinear regression analysis using InPlot 4.0.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
L162389 Related Classifications
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