AcdK
AcdK is a non-natural amino acid and a precursor of allysine. AcdK allows site-specific incorporation into target proteins in E. coli via the amber suppression strategy. AcdK enables site-specific lysine dimethylation or monomethylation modification of target proteins. AcdK can synthesize site-specific lysine-methylated variants of histone H3 and p53, which is applicable for investigating the substrate specificity and catalytic function of epigenetic enzymes.
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- No. CAS: 2097535-26-5
- Fòrmula: C14H17N5O4
- Peso molecular:319.32
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
Descripciòn
In Vitro
AcdK (0.5 mM) is efficiently and site-specifically incorporated into sfGFP at position D134 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable quantitative conversion to sfGFP with site-specific lysine dimethylation at position D134[1].
AcdK (2 mM) is efficiently and site-specifically incorporated into histone H3 at positions K4, K9, or K36 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable conversion to site-specific dimethylated histone H3 variants[1].
AcdK is efficiently and site-specifically incorporated into p53 at position K372 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable conversion to p53 with site-specific lysine mono- or dimethylation at K372, which directly enhances Tip60-catalyzed acetylation of p53 at K120 by 2.5- to 3-fold[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2097535-26-5
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Peso molecular 319.32
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Fòrmula C14H17N5O4
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SMILES
OC([C@@H](N)CC/C=C/NC(OCC1=CC=C(C=C1)N=[N+]=[N-])=O)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AcdK Related Classifications
HY-186091 Related Classifications
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