AMG7703
Based on 1 Customer Validation
AMG7703 is a selective and allosteric agonists of FFA2 (GPR43), the receptor for short-chain fatty acids (SCFAs), acetate, and propionate. AMG7703 can be used to research for in inflammatory and metabolic.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.92%
- No. CAS: 1103523-24-5
- Fòrmula: C14H15ClN2OS
- Peso molecular:294.80
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
0.7 μM
Compound: 2
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Agonist activity at human FFA2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
Agonist activity at human FFA2 receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP production
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[PMID: 20005104] |
In Vitro
AMG7703 (phenylacetamide 1) (30 μM) shows selectivity on FFA2 (GPR43) over FFA1 (GPR40) and FFA3 (GPR41) with in Chinese hamster ovary cells[1].
AMG7703 (0.041, 0.123, 0.37, 1.11 μM) acts as an FFA2 allosteric agonist, activates Gαi coupled signaling pathway[1].
AMG7703 (1, 3, 10, 30 μM) results in Gαi-dependent inhibition of lipolysis in adipocytes (3T3L1)[1].
AMG7703 (0.041, 0.123, 0.37, 1.11 μM) exhibits allosteric activity and inhibits Gαi-coupled cAMP with IC50 values of 0.7 μM (hFFA2) and 0.96 μM (mFFA2), respectively; as for Gαq-coupled aequorin inhibition, with EC50s of 0.45 μM (hFFA2) and 1.27 μM (mFFA2), respectively[1].
AMG7703 (0-1 μM) shows the positive cooperating effect of acetate and stimulates calcium mobilization in a concentration-dependent manner in CHO cell stably expressing hFFA2 and aequorin[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 1103523-24-5
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Appearance Solid
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Peso molecular 294.80
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Fòrmula C14H15ClN2OS
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Color White to off-white
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SMILES
O=C(NC1=NC=CS1)[C@H](C2=CC=C(Cl)C=C2)C(C)C
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (339.21 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3921 mL | 16.9607 mL | 33.9213 mL | 84.8033 mL |
| 5 mM | 0.6784 mL | 3.3921 mL | 6.7843 mL | 16.9607 mL | |
| 10 mM | 0.3392 mL | 1.6961 mL | 3.3921 mL | 8.4803 mL | |
| 15 mM | 0.2261 mL | 1.1307 mL | 2.2614 mL | 5.6536 mL | |
| 20 mM | 0.1696 mL | 0.8480 mL | 1.6961 mL | 4.2402 mL | |
| 25 mM | 0.1357 mL | 0.6784 mL | 1.3569 mL | 3.3921 mL | |
| 30 mM | 0.1131 mL | 0.5654 mL | 1.1307 mL | 2.8268 mL | |
| 40 mM | 0.0848 mL | 0.4240 mL | 0.8480 mL | 2.1201 mL | |
| 50 mM | 0.0678 mL | 0.3392 mL | 0.6784 mL | 1.6961 mL | |
| 60 mM | 0.0565 mL | 0.2827 mL | 0.5654 mL | 1.4134 mL | |
| 80 mM | 0.0424 mL | 0.2120 mL | 0.4240 mL | 1.0600 mL | |
| 100 mM | 0.0339 mL | 0.1696 mL | 0.3392 mL | 0.8480 mL |
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