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Referencia número: HY-D3210

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DCM-KPV

DCM-KPV is a fluorescent probe targeting the human intestinal oligopeptide transporter PEPT1/SLC15A1 receptor (λ_ex=480 nm, λ_em=620-670 nm). DCM-KPV specifically binds to PepT1 via its KPV domain and mediates receptor-targeted internalization, thus effectively accumulating in the cytoplasm and nucleus of cells overexpressing this receptor. DCM-KPV has the advantages of long emission wavelength, high emission efficiency, low photobleaching, and negligible cytotoxicity. DCM-KPV maintains stable fluorescence intensity under continuous illumination, exhibiting extremely high live cell compatibility. DCM-KPV can specifically accumulate at colonic inflammatory sites through the intestinal mucosa, enabling direct non-invasive visual differentiation between chronic and acute ulcerative colitis groups and the normal group.

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No. CAS: 2098632-04-1
Fòrmula: C₄₀H₄₈N₈O₄
Peso molecular:704.86

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

DCM-KPV Chemical Structure

No. CAS: 2098632-04-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- In Vitro
- In Vivo
Chemical Information
Pureza y Documentación
Referencias
Calculators
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Actividad biológica

Descripciòn

In Vitro

DCM-KPV (1-30 μM; 24-72 h) exhibits negligible cytotoxicity and high cytocompatibility with Caco-2 cells^[2].
DCM-KPV (1.0 μM; 50 min) is efficiently internalized into Caco-2 cells via PepT1 receptor-mediated uptake, accumulating in the cytoplasm and nucleus^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:

PepT1-overexpressed Caco-2 human colon adenocarcinoma cells
Concentration:

1-30 μM (viability assays); 1-30 μM (apoptosis assays)
Incubation Time:

24-72 h (viability assays); 24 h (apoptosis assays)
Result:

Maintained >95% cell viability at all tested concentrations and incubation times.
Showed no significant differences in cell proliferation rate or cell growth inhibition rate relative to controls after 24, 48, and 72 h of incubation.
Showed no appreciable increase in apoptosis rate relative to controls after 24 h of incubation.

In Vivo

DCM-KPV (2.5 mg/kg; via anus; single dose; reserved for 1 hour) achieves noninvasive visualization of ulcerative colitis in Mus musculus (mice), with significantly higher fluorescence accumulation in chronic colitis than acute colitis and normal colon tissues, enabling discrimination between chronic, acute, and normal groups^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c mice with Ulcerative colitis (male, 6-8-week-old)^[2]
Dosage:

2.5 mg/kg
Administration:

via anus; single dose; reserved for 1 hour
Result:

Reached an average fluorescence intensity of 9.33E+08 a.u. in distal colon tissues of chronic colitis mice.
Reached an average fluorescence intensity of 4.08E+08 a.u. in distal colon tissues of acute colitis mice.
Reached an average fluorescence intensity of 2.25E+08 a.u. in distal colon tissues of normal control mice.

Chemical Information

No. CAS 2098632-04-1
Peso molecular 704.86
Fòrmula C₄₀H₄₈N₈O₄
SMILES

CC(C(C(N1CCN(CC1)C2=CC=C(C=C2)/C=C/C3=C/C(C4=CC=CC=C4O3)=C(C#N)/C#N)=O)NC(C5CCCN5C(C(CCCCN)N)=O)=O)C
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureza y Documentación

Instrucciones de manejo (2659 KB)

Referencias

[1]. Wang B, et al. A Review on Recent Advances in Peptide‐based Fluorescence Probes and their Potential Applications[J]. ChemistrySelect, 2023, 8(31): e202302216.

[2]. Zeng M, et al. Peptide Receptor-Targeted Fluorescent Probe: Visualization and Discrimination between Chronic and Acute Ulcerative Colitis. ACS Appl Mater Interfaces. 2017;9(15):13029-13036. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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