ER-Tracker Green (solution)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of ER-Tracker working solution
1.1 Preparation of the stock solution
Stock solution.
It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of ER-Tracker working solution
Dilute the stock solution in serum-free cell culture medium or PBS. The corresponding stock solution can be diluted according to the actual situation. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
Note: Please adjust the concentration of ER-Tracker working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
2.1.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
2.1.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
2.1.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.1.4 Wash twice with PBS, 5 minutes each time.
2.1.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
2.2.1 Culture adherent cells on sterile coverslips.
2.2.2 Remove the coverslip from the medium and aspirate excess medium.
2.2.3 Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
2.2.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.