Exo2
Based on 1 Customer Validation
Exo2 is a secretion inhibitor. Exo2 perturbs trafficking of Shiga toxin between endosomes and the trans-Golgi network. Exo2 blocks secretory cargo exit from the ER (endoplasmic reticulum) and disrupts the Golgi apparatus, but does not affect the morphology of the TGN (trans-Golgi network) Exo2 can stimulate calcium-dependent exocytosis in permeabilized adrenal chromaff in cells.
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- Pureza : 99.57%
- No. CAS: 304684-77-3
- Fòrmula: C18H18N4O2S
- Peso molecular:354.43
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| DU-145 | GI50 |
9.9 μM
Compound: 15
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Cytotoxicity against human DU145 cells
Cytotoxicity against human DU145 cells
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[PMID: 20356752] |
| HT-29 | GI50 |
18.7 μM
Compound: 15
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Cytotoxicity against human HT-29 cells
Cytotoxicity against human HT-29 cells
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[PMID: 20356752] |
| K562 | GI50 |
9 μM
Compound: 15
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Cytotoxicity against human K562 cells
Cytotoxicity against human K562 cells
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[PMID: 20356752] |
| M14 | GI50 |
18.8 μM
Compound: 15
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Cytotoxicity against human M14 cells
Cytotoxicity against human M14 cells
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[PMID: 20356752] |
| Macrophage | IC50 |
23.8 μM
Compound: 15
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Cytotoxicity against BALB/c mouse macrophages after 48 hrs by trypan blue dye exclusion method
Cytotoxicity against BALB/c mouse macrophages after 48 hrs by trypan blue dye exclusion method
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[PMID: 20356752] |
| MCF7 | GI50 |
13.6 μM
Compound: 15
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Cytotoxicity against human MCF7 cells
Cytotoxicity against human MCF7 cells
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[PMID: 20356752] |
| NCI-H460 | GI50 |
20.3 μM
Compound: 15
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Cytotoxicity against human NCI-H460 cells
Cytotoxicity against human NCI-H460 cells
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[PMID: 20356752] |
| NIH3T3 | GI50 |
10.2 μM
Compound: 15
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Cytotoxicity against BALB mouse 3T3 cells
Cytotoxicity against BALB mouse 3T3 cells
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[PMID: 20356752] |
| Vero | IC50 |
11.3 μM
Compound: 15
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Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS assay
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS assay
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[PMID: 20356752] |
| Vero | IC50 |
17.5 μM
Compound: 15
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Antitrypanosomal activity against Trypanosoma cruzi tulahuen C4 trypomastigotes infected in african green monkey Vero cells assessed as CPRG cleavage after 120 hrs by microplate reader
Antitrypanosomal activity against Trypanosoma cruzi tulahuen C4 trypomastigotes infected in african green monkey Vero cells assessed as CPRG cleavage after 120 hrs by microplate reader
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[PMID: 20356752] |
Chemical Information
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No. CAS 304684-77-3
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Appearance Solid
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Peso molecular 354.43
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Fòrmula C18H18N4O2S
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Color Light yellow to yellow
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SMILES
COC(C=C(C=C1)/C=N/NC2=C3C(CCCC4)=C4SC3=NC=N2)=C1O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 25 mg/mL (70.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Spooner RA, et al. The secretion inhibitor Exo2 perturbs trafficking of Shiga toxin between endosomes and the trans-Golgi network. Biochem J. 2008 Sep 15;414(3):471-84. [Content Brief]
[2]. Morgan A, et al. Exo1 and Exo2 proteins stimulate calcium-dependent exocytosis in permeabilized adrenal chromaffin cells. Nature. 1992 Feb 27;355(6363):833-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8214 mL | 14.1072 mL | 28.2143 mL | 70.5358 mL |
| 5 mM | 0.5643 mL | 2.8214 mL | 5.6429 mL | 14.1072 mL | |
| 10 mM | 0.2821 mL | 1.4107 mL | 2.8214 mL | 7.0536 mL | |
| 15 mM | 0.1881 mL | 0.9405 mL | 1.8810 mL | 4.7024 mL | |
| 20 mM | 0.1411 mL | 0.7054 mL | 1.4107 mL | 3.5268 mL | |
| 25 mM | 0.1129 mL | 0.5643 mL | 1.1286 mL | 2.8214 mL | |
| 30 mM | 0.0940 mL | 0.4702 mL | 0.9405 mL | 2.3512 mL | |
| 40 mM | 0.0705 mL | 0.3527 mL | 0.7054 mL | 1.7634 mL | |
| 50 mM | 0.0564 mL | 0.2821 mL | 0.5643 mL | 1.4107 mL | |
| 60 mM | 0.0470 mL | 0.2351 mL | 0.4702 mL | 1.1756 mL |
Exo2 Related Classifications
HY-137975 Related Classifications
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