SHP389
Based on 1 Customer Validation
SHP389 is an allosteric SHP2 inhibitor, with an IC50 of 36 nM for both SHP2 and p-ERK.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.03%
- No. CAS: 2235394-90-6
- Fòrmula: C23H29ClN8O2
- Peso molecular:484.98
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
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Help & FAQs
Actividad biológica
IC50: 36 nM (SHP2 and p-ERK)[1].
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Detroit 562 | IC50 |
4.13 μM
Compound: SHP389
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Antiproliferative activity against human Detroit 562 cells after 24 hrs by Celltiter-Glo assay
Antiproliferative activity against human Detroit 562 cells after 24 hrs by Celltiter-Glo assay
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[PMID: 30688459] |
| KYSE-520 cell line | IC50 |
0.36 μM
Compound: SHP389; 1
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Antiproliferative activity against human KYSE520 cells after 24 hrs by Celltiter-Glo reagent-based assay
Antiproliferative activity against human KYSE520 cells after 24 hrs by Celltiter-Glo reagent-based assay
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[PMID: 30688462] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2235394-90-6
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Appearance Solid
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Peso molecular 484.98
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Fòrmula C23H29ClN8O2
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Color White to off-white
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SMILES
O=C1C2=C(C3=C(Cl)C(NC4CC4)=NC=C3)NN=C2N=C(N(CC5)CCC65[C@H](N)[C@H](C)OC6)N1C
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (206.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.31 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (249 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0619 mL | 10.3097 mL | 20.6194 mL | 51.5485 mL |
| 5 mM | 0.4124 mL | 2.0619 mL | 4.1239 mL | 10.3097 mL | |
| 10 mM | 0.2062 mL | 1.0310 mL | 2.0619 mL | 5.1549 mL | |
| 15 mM | 0.1375 mL | 0.6873 mL | 1.3746 mL | 3.4366 mL | |
| 20 mM | 0.1031 mL | 0.5155 mL | 1.0310 mL | 2.5774 mL | |
| 25 mM | 0.0825 mL | 0.4124 mL | 0.8248 mL | 2.0619 mL | |
| 30 mM | 0.0687 mL | 0.3437 mL | 0.6873 mL | 1.7183 mL | |
| 40 mM | 0.0515 mL | 0.2577 mL | 0.5155 mL | 1.2887 mL | |
| 50 mM | 0.0412 mL | 0.2062 mL | 0.4124 mL | 1.0310 mL | |
| 60 mM | 0.0344 mL | 0.1718 mL | 0.3437 mL | 0.8591 mL | |
| 80 mM | 0.0258 mL | 0.1289 mL | 0.2577 mL | 0.6444 mL | |
| 100 mM | 0.0206 mL | 0.1031 mL | 0.2062 mL | 0.5155 mL |
SHP389 Related Classifications
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