TJ191
Based on 1 Customer Validation
TJ191 is a potent and specific anti-cancer agent that targets low TβRIII-expressing malignant T-cell leukemia/lymphoma cells. TJ191 has no affects on the proliferation of other cancer cells or normal fibroblasts or immune cells. TJ191 can be used for cancer research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.83%
- No. CAS: 1522415-97-9
- Fòrmula: C13H21NO2S
- Peso molecular:255.38
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CAKI-1 | IC50 |
0.54 μM
Compound: 3j
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Cytotoxicity against human Caki1 cells after 72 to 96 hrs by coulter counter method
Cytotoxicity against human Caki1 cells after 72 to 96 hrs by coulter counter method
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[PMID: 28365318] |
| CCRF-CEM | IC50 |
>=34 μM
Compound: 3j
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Antiproliferative activity against human CEM cells passaged every 4 to 5 days in presence of compound up to 10 uM
Antiproliferative activity against human CEM cells passaged every 4 to 5 days in presence of compound up to 10 uM
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[PMID: 28365318] |
| CCRF-CEM | IC50 |
0.18 μM
Compound: 3j
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Cytotoxicity against human CEM cells after 72 to 96 hrs by coulter counter method
Cytotoxicity against human CEM cells after 72 to 96 hrs by coulter counter method
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[PMID: 28365318] |
| CCRF-CEM | IC50 |
0.9 μM
Compound: 8
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Antiproliferative activity against human CEM cells assessed as reduction in cell viability
Antiproliferative activity against human CEM cells assessed as reduction in cell viability
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[PMID: 28987607] |
| HeLa | IC50 |
81 μM
Compound: 3j
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Cytotoxicity against human HeLa cells after 72 to 96 hrs by coulter counter method
Cytotoxicity against human HeLa cells after 72 to 96 hrs by coulter counter method
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[PMID: 28365318] |
| Huh-7 | IC50 |
0.34 μM
Compound: 3j
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Cytotoxicity against human HuH7 cells after 72 to 96 hrs by coulter counter method
Cytotoxicity against human HuH7 cells after 72 to 96 hrs by coulter counter method
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[PMID: 28365318] |
| MOLT-4 | IC50 |
0.057 μM
Compound: 3j
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Cytotoxicity against human MOLT4 cells after 72 to 96 hrs by coulter counter method
Cytotoxicity against human MOLT4 cells after 72 to 96 hrs by coulter counter method
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[PMID: 28365318] |
| PBMC | IC50 |
>50 μM
Compound: 3j
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Cytotoxicity against human PBMC cells after 72 to 96 hrs by coulter counter method
Cytotoxicity against human PBMC cells after 72 to 96 hrs by coulter counter method
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[PMID: 28365318] |
| PC-3 | IC50 |
>=34 μM
Compound: 3j
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Antiproliferative activity against human PC3 cells passaged every 4 to 5 days in presence of compound up to 10 uM
Antiproliferative activity against human PC3 cells passaged every 4 to 5 days in presence of compound up to 10 uM
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[PMID: 28365318] |
| PC-3 | IC50 |
0.62 μM
Compound: 3j
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Cytotoxicity against human PC3 cells after 72 to 96 hrs by coulter counter method
Cytotoxicity against human PC3 cells after 72 to 96 hrs by coulter counter method
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[PMID: 28365318] |
| Raji | IC50 |
105 μM
Compound: 3j
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Cytotoxicity against human Raji cells after 72 to 96 hrs by coulter counter method
Cytotoxicity against human Raji cells after 72 to 96 hrs by coulter counter method
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[PMID: 28365318] |
In Vitro
TJ191(0-100 μM; 24 hours) exhibits pronounced anti-proliferative activity in malignant cell lines, the IC50 values are 0.13 μM, 0.13±0.08 μM, 0.26±0.19 μM, 0.22±0.11 μM, 1.5±0.02 μM, 0.32±0.086 μM, 3.1±0.5 μM, 0.26±0.16 μM in CEM, JURKAT, MOLT-3, MOLT-4, SUP-T1, MT-2, C8166 and HSB-2 cells, respectively. But has no effects in HUT-78 (IC50=17±10 μM) and MT-4 (IC50=47 ± 5 μM) cells[1].TJ191 (0.1-3 μM; 8 or 24 hours) induces CEM cell apoptosis in a concentration- and time-dependent manner. Even at 0.3 μM, TJ191 induces the maximum apoptotic rate of 80% after 24 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Malignant T-cell leukemia/lymphoma cells
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Concentration:0.01 µM, 0.1 µM, 1 µM, 10 µM, 100 µM
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Incubation Time:24 hours
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Result:Exhibited inhibitory effects in drug-sensitive cells with IC50 ranging 0.13 µM to 3.1 µM.
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Cell Line:CEM cell line
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Concentration:0.1-3 µM
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Incubation Time:8 or 24 hours
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Result:Led to cell apoptosis.
Chemical Information
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No. CAS 1522415-97-9
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Appearance Solid
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Peso molecular 255.38
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Fòrmula C13H21NO2S
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Color Light yellow to khaki
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SMILES
O=C(C1=C(N)SC(CCCCCCC)=C1)OC
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (391.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.79 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9157 mL | 19.5787 mL | 39.1573 mL | 97.8933 mL |
| 5 mM | 0.7831 mL | 3.9157 mL | 7.8315 mL | 19.5787 mL | |
| 10 mM | 0.3916 mL | 1.9579 mL | 3.9157 mL | 9.7893 mL | |
| 15 mM | 0.2610 mL | 1.3052 mL | 2.6105 mL | 6.5262 mL | |
| 20 mM | 0.1958 mL | 0.9789 mL | 1.9579 mL | 4.8947 mL | |
| 25 mM | 0.1566 mL | 0.7831 mL | 1.5663 mL | 3.9157 mL | |
| 30 mM | 0.1305 mL | 0.6526 mL | 1.3052 mL | 3.2631 mL | |
| 40 mM | 0.0979 mL | 0.4895 mL | 0.9789 mL | 2.4473 mL | |
| 50 mM | 0.0783 mL | 0.3916 mL | 0.7831 mL | 1.9579 mL | |
| 60 mM | 0.0653 mL | 0.3263 mL | 0.6526 mL | 1.6316 mL | |
| 80 mM | 0.0489 mL | 0.2447 mL | 0.4895 mL | 1.2237 mL | |
| 100 mM | 0.0392 mL | 0.1958 mL | 0.3916 mL | 0.9789 mL |
TJ191 Related Classifications
HY-120075 Related Classifications
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