Gluten Exorphin A5

Gluten Exorphin A5 (10-100 μg; 2-6 h) exerts no cytotoxic effect on 70% Caco-2/30% HT-29 co-cultured cells after 2 h or 6 h of incubation^[3].
Gluten Exorphin A5 (30 μg; 30 or 120 min) passes through the 70% Caco-2/30% HT-29 intestinal epithelial co-culture layer as an intact peptide, with basolateral recovery ratios accounting for 0.5% and 3% of the apical administered dose, respectively. The P_app reaches 2.06 × 10^-6 cm/s at 120 min of exposure^[3].
After 2 h of incubation with Gluten Exorphin A5 (30-600 μg; 2 h), the 600 μg concentration increases the viability of 70% Caco2/30% HT-29 co-cultured cells by 20%-30%, while the 30 μg and 150 μg doses exert no significant effects^[2].
Gluten Exorphin A5 (30-600 μg; 120 min) reduces the TEER of 70% Caco2/30% HT-29 co-cultured monolayers in a dose-dependent manner. After 120 min of incubation, the 600 μg dose decreases TEER by 30%, while the 30 μg dose shows no significant effect^[2].
The P_app of Gluten Exorphin A5 (600 μg; 120 min) across the 70% Caco-2/30% HT-29 intestinal epithelial co-culture layer is 19.53 × 10^-6 cm/s; it increases the viability of 70% Caco-2/30% HT-29 co-cultured cells by approximately 20-30%, while 30 μg and 150 μg exert no significant effects^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.