Guinea pig liver transglutaminase
Guinea pig liver transglutaminase (Tissue-type transglutaminase) is a calcium-dependent tissue-type transglutaminase and serves as the prototype of intracellular tissue transglutaminases purified from guinea pig liver. Guinea pig liver transglutaminase catalyzes acyl transfer reactions via a ping-pong mechanism, forming a transient thioester acyl-enzyme intermediate that mediates the hydrolysis of glutamine amides, displacement by primary amines, and the formation of isopeptide cross-links between peptide-bound glutamine and the amino groups of lysine or polyamines.
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- CAS No.: 80146-85-6
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Guinea pig liver transglutaminase is irreversibly inactivated by Iodoacetamide (2-Iodoacetamide) (HY-34477) in a Ca2+-dependent manner through alkylation of a single essential cysteine residue at the active site, which is protected by substrate[1].
Multiple thiol reagents inhibit guinea pig liver transglutaminase, with Ca2+-dependent reactivity of an essential active-site cysteine demonstrated for NEM (N-Ethylmaleimide) (HY-D0843), and both reversible and irreversible inhibition patterns observed for CMB and DTNB (HY-15915)[1].
Guinea pig liver transglutaminase binds specifically to GTP-agarose (but not CTP-agarose), and this binding is inhibited by the presence of 10 mM calcium chloride[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 80146-85-6
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SMILES
[Guinea pig liver transglutaminase]
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Synonyms
Tissue-type transglutaminase; TG2; TGase 2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Guinea pig liver transglutaminase Related Classifications
HY-E70437 Related Classifications
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Keywords
- Guinea pig liver transglutaminase
- 80146-85-6
- Tissue-type transglutaminase
- TG2
- TGase 2
- TG2
- TG 2
- TG-2
- TGase2
- TGase 2
- TGase-2
- Biochemical Assay Reagents
- calcium ion
- extracellular matrix stabilization
- iodoacetamide
- human tissue transglutaminase
- guinea pig liver transglutaminase
- Huntington's disease
- Alzheimer's disease
- tissue-type transglutaminase
- wound healing
- GTP
- Inhibitor
- inhibitor
- inhibit