Hedgehog agonist 1
Based on 1 Customer Validation
Hedgehog agonist 1 is a Hedgehog agonist that activates Hedgehog signaling by binding to Smoothened. Hedgehog agonist 1 can be used for research on stroke.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.78%
- No. CAS: 946002-48-8
- Fòrmula: C29H28ClF2N3O2S
- Peso molecular:556.07
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
In Vitro
Hedgehog agonist 1 (21k) has an EC50 of 0.3 nM and Amax of 80% in mouse cell lines using a Gli-luc reporter assay[1].
Hedgehog agonist 1 (21k) has an EC50 of 0.4 nM and Amax of 86% in daoy human cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 946002-48-8
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Appearance Solid
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Peso molecular 556.07
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Fòrmula C29H28ClF2N3O2S
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Color Off-white to light yellow
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SMILES
O=C(C1=C(Cl)C2=C(F)C=CC(F)=C2S1)N(CC3=CC(C4=CC=NC=C4)=CC=C3OC)[C@H]5CC[C@H](NC)CC5
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 40 mg/mL (71.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.50 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (294 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7983 mL | 8.9917 mL | 17.9833 mL | 44.9584 mL |
| 5 mM | 0.3597 mL | 1.7983 mL | 3.5967 mL | 8.9917 mL | |
| 10 mM | 0.1798 mL | 0.8992 mL | 1.7983 mL | 4.4958 mL | |
| 15 mM | 0.1199 mL | 0.5994 mL | 1.1989 mL | 2.9972 mL | |
| 20 mM | 0.0899 mL | 0.4496 mL | 0.8992 mL | 2.2479 mL | |
| 25 mM | 0.0719 mL | 0.3597 mL | 0.7193 mL | 1.7983 mL | |
| 30 mM | 0.0599 mL | 0.2997 mL | 0.5994 mL | 1.4986 mL | |
| 40 mM | 0.0450 mL | 0.2248 mL | 0.4496 mL | 1.1240 mL | |
| 50 mM | 0.0360 mL | 0.1798 mL | 0.3597 mL | 0.8992 mL | |
| 60 mM | 0.0300 mL | 0.1499 mL | 0.2997 mL | 0.7493 mL |
Hedgehog agonist 1 Related Classifications
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