Hedgehog agonist 1

Name: Hedgehog agonist 1
Brand: MedChemExpress
SKU: HY-111040
Rating: 4.5 (1 reviews)

Cat. No.: HY-111040 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Hh agonist 1 (compound 21k) is a potent Hedgehog (Hh) agonist, with an EC₅₀ 0.3 nM. Hh agonist 1 can be used for the research of stroke and other neurological disorders.

For research use only. We do not sell to patients.

Hedgehog agonist 1 Chemical Structure

CAS No. : 946002-48-8

Size	Price	Stock	Quantity
5 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 525	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 890	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1750	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
Purity & Documentation
References
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Description

Hh agonist 1 (compound 21k) is a potent Hedgehog (Hh) agonist, with an EC₅₀ 0.3 nM. Hh agonist 1 can be used for the research of stroke and other neurological disorders^[1].

IC₅₀ & Target

EC50: 0.3 nM (Hh)^[1].

Molecular Weight

556.07

Formula

C₂₉H₂₈ClF₂N₃O₂S

CAS No.

946002-48-8

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=C(Cl)C2=C(F)C=CC(F)=C2S1)N(CC3=CC(C4=CC=NC=C4)=CC=C3OC)[C@H]5CC[C@H](NC)CC5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 40 mg/mL (71.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7983 mL	8.9917 mL	17.9833 mL
5 mM	0.3597 mL	1.7983 mL	3.5967 mL
10 mM	0.1798 mL	0.8992 mL	1.7983 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (278 KB) SDS (0 KB)

COA (259 KB) HNMR (303 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Brunton SA, et al. Potent agonists of the Hedgehog signaling pathway. Bioorg Med Chem Lett. 2009 Aug 1;19(15):4308-11. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7983 mL	8.9917 mL	17.9833 mL	44.9584 mL
	5 mM	0.3597 mL	1.7983 mL	3.5967 mL	8.9917 mL
	10 mM	0.1798 mL	0.8992 mL	1.7983 mL	4.4958 mL
	15 mM	0.1199 mL	0.5994 mL	1.1989 mL	2.9972 mL
	20 mM	0.0899 mL	0.4496 mL	0.8992 mL	2.2479 mL
	25 mM	0.0719 mL	0.3597 mL	0.7193 mL	1.7983 mL
	30 mM	0.0599 mL	0.2997 mL	0.5994 mL	1.4986 mL
	40 mM	0.0450 mL	0.2248 mL	0.4496 mL	1.1240 mL
	50 mM	0.0360 mL	0.1798 mL	0.3597 mL	0.8992 mL
	60 mM	0.0300 mL	0.1499 mL	0.2997 mL	0.7493 mL