Clopamide
Based on 1 publication(s) in Google Scholar
Clopamide is an orally active thiazide-like diuretic agent that inhibits the sodium-coupled chloride cotransporter SLC12A3. Clopamide has the potential for hypertension and cardiac failure research.
For research use only. We do not sell to patients.
- Purity: 99.0%
- CAS No.: 636-54-4
- Formula: C14H20ClN3O3S
- Molecular Weight:345.84
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Cardiovascular Disease Compound Library
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Publications Citing Use of MedChemExpress (MCE) Clopamide
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Biological Activity
Clopamide is actively secreted by renal tubular cells and the True Tubular Excretion Fraction (TTEF) value is 10%[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 636-54-4
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Appearance Solid
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Molecular Weight 345.84
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Formula C14H20ClN3O3S
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Color White to yellow
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SMILES
O=C(NN1[C@H](C)CCC[C@@H]1C)C2=CC=C(Cl)C(S(=O)(N)=O)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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J Pharm Biomed Anal
Screening method of mildronate and over 300 doping agents by reversed-phase liquid chromatography-high resolution mass spectrometry. [Abstract]2021 Feb 20:195:113870. PMID: 33453569
Solvent & Solubility
DMSO : 100 mg/mL (289.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. J J McNeil, et al. Clopamide: plasma concentrations and diuretic effect in humans. Clin Pharmacol Ther. 1987 Sep;42(3):299-304. [Content Brief]
[2]. Yoshiteru Noutoshi, et al. Diuretics prime plant immunity in Arabidopsis thaliana. PLoS One. 2012;7(10):e48443. [Content Brief]
[3]. B Odlind, et al. Renal tubular secretion and effects of chlorothiazide, hydrochlorothiazide and clopamide: a study in the avian kidney. Acta Pharmacol Toxicol (Copenh). 1982 Sep;51(3):187-97. [Content Brief]
[4]. E Müller-Schweinitzer, et al. Interaction of cyclosporine-A with the renin-angiotensin system in canine veins. Naunyn Schmiedebergs Arch Pharmacol. 1989 Aug;340(2):252-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.8915 mL | 14.4576 mL | 28.9151 mL | 72.2878 mL |
| 5 mM | 0.5783 mL | 2.8915 mL | 5.7830 mL | 14.4576 mL | |
| 10 mM | 0.2892 mL | 1.4458 mL | 2.8915 mL | 7.2288 mL | |
| 15 mM | 0.1928 mL | 0.9638 mL | 1.9277 mL | 4.8192 mL | |
| 20 mM | 0.1446 mL | 0.7229 mL | 1.4458 mL | 3.6144 mL | |
| 25 mM | 0.1157 mL | 0.5783 mL | 1.1566 mL | 2.8915 mL | |
| 30 mM | 0.0964 mL | 0.4819 mL | 0.9638 mL | 2.4096 mL | |
| 40 mM | 0.0723 mL | 0.3614 mL | 0.7229 mL | 1.8072 mL | |
| 50 mM | 0.0578 mL | 0.2892 mL | 0.5783 mL | 1.4458 mL | |
| 60 mM | 0.0482 mL | 0.2410 mL | 0.4819 mL | 1.2048 mL | |
| 80 mM | 0.0361 mL | 0.1807 mL | 0.3614 mL | 0.9036 mL | |
| 100 mM | 0.0289 mL | 0.1446 mL | 0.2892 mL | 0.7229 mL |
Clopamide Related Classifications
HY-B1477 Related Classifications
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