1. Cell Cycle/DNA Damage
    Epigenetics
    Metabolic Enzyme/Protease
  2. HDAC
    MMP
    HIF/HIF Prolyl-Hydroxylase
  3. HNHA

HNHA 

Cat. No.: HY-118672
Handling Instructions

HNHA is a potent histone deacetylase (HDAC) inhibitor. HNHA arrests the cell cycle at the G1/S phase via p21 induction. HNHA inhibits tumor growth and tumor neovascularization. HNHA may be a potent anti-cancer agent against breast cancer.

For research use only. We do not sell to patients.

HNHA Chemical Structure

HNHA Chemical Structure

CAS No. : 926908-04-5

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Description

HNHA is a potent histone deacetylase (HDAC) inhibitor. HNHA arrests the cell cycle at the G1/S phase via p21 induction. HNHA inhibits tumor growth and tumor neovascularization. HNHA may be a potent anti-cancer agent against breast cancer[1].

IC50 & Target

MMP-2

 

MMP-9

 

In Vitro

HNHA (0-100 μM, 96 h) shows strong inhibition at lower concentrations on cancer cell lines, especially on breast cancer cells, mouse FM3A and human MCF-7[1].
HNHA (15 μM, 24 h) arrests cancer cells at the G1/S phase of the cell cycle, activates p21and rescues strongly protein acetylation[1].
HNHA (15 μM, 12 h) inhibits angiogenic proteins in breast cancer cells, effectively inactivates MMP-2, MMP-9, VEGF and HIF-1α[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line: FM3A, C1300, LA-N-1, LA-N-2, LA-N-5, NB16, NB19, NB69, SK-N-SH, MCF-7 and HT-29[1]
Concentration: 0-100 μM
Incubation Time: 96 h
Result: Showed strong inhibition at lower concentrations on all cancer cell lines (FM3A, C1300, LA-N-1, LA-N-2, LA-N-5, NB16, NB19, NB69, SK-N-SH, MCF-7 and HT-29), with IC50 values of 15.70, 55.63, 22.78, 23.18, 26.70, 19.64, 21.26, 22.31, 65.09, 14.33, and 16.98 μM, respectively.

Cell Viability Assay

Cell Line: FM3A and MCF-7[1]
Concentration: 0, 0.1, 1, 5, 10, 15, 20, 25,30 μM
Incubation Time: 48 h
Result: Showed dose-dependent inhibition of viability in mouse and human breast cancer cells.

Cell Cycle Analysis

Cell Line: FM3A and MCF-7 cells[1]
Concentration: 15 μM
Incubation Time: 24 h
Result: Arrested FM3A and MCF-7 cells in the G1/S phase.

Western Blot Analysis

Cell Line: FM3A and MCF-7 cells[1]
Concentration: 0, 0.1, 1, 10, and 20 μM (24 h)
Incubation Time: 1, 6, 24, 48, and 72 h (15 μM)
Result: Activated a cell proliferation arrestor p21, increased histone and non-histone protein acetylation and inhibited FM3A and MCF-7 proliferation in vitro, and was very effective in increasing the acetylation level of histone H3 protein in FM3A and MCF-7. The most effective dose point for acetylation of histone H3 was 10-20 μM. Histone H3 acetylation peaked after 1 h of exposure to the drugs and remained stable for 1-6 h.

Western Blot Analysis

Cell Line: FM3A and MCF-7 cells[1]
Concentration: 15 μM
Incubation Time: 12 h
Result: Showed a strong induction of TIMP-1 and TIMP-2, and effectively inactivated MMP-2, MMP-9, VEGF and HIF-1α.
In Vivo

HNHA (20 μM/mouse, IP, once every 2 days for a total of six injections) reduces tumor burden and extends the survival rate, activates TIMP-1, TIMP-2 and p21 and inhibits MMP-2, MMP-9, HIF-1α and VEGF protein expression[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C3H/HeJ-FasL mice (FM3A breast cancer cell tumor xenograft, 6 weeks, n = 25/group)[1]
Dosage: 20 μM/mouse
Administration: IP, once every 2 days for a total of six injections
Result: Reduced tumor burden and extended the survival rate. Effectively inhibited cancer development and angiogenesis in vivo. Increased TIMP-1, TIMP-2 and p21, decreased MMP-2, MMP-9, HIF-1α and VEGF protein expression, and reduced the distribution of CD34, HIF-1α and VEGF.
Molecular Weight

303.42

Formula

C17H21NO2S

CAS No.
SMILES

O=C(NO)CCCCCCSC1=CC=C2C=CC=CC2=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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