1. Apoptosis
  2. c-Myc
    Apoptosis
  3. hnRNPK-IN-1

hnRNPK-IN-1 

Cat. No.: HY-135691
Handling Instructions

hnRNPK-IN-1 is a heterogeneous nuclear ribonucleoprotein K (hnRNPK) binding ligand with Kd values of 4.6 μM and 2.6 μM measured with SPR and MST, respectively. hnRNPK-IN-1 inhibits c-myc transcription by disrupting the binding of hnRNPK and c-myc promoter. hnRNPK-IN-1 induces Hela cells apoptosis and has strongly anti-tumor activities.

For research use only. We do not sell to patients.

hnRNPK-IN-1 Chemical Structure

hnRNPK-IN-1 Chemical Structure

CAS No. : 2313528-04-8

Size Price Stock Quantity
5 mg USD 550 In-stock
Estimated Time of Arrival: December 31
10 mg USD 880 In-stock
Estimated Time of Arrival: December 31
25 mg USD 1800 In-stock
Estimated Time of Arrival: December 31
50 mg USD 3000 In-stock
Estimated Time of Arrival: December 31
100 mg USD 4600 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

hnRNPK-IN-1 is a heterogeneous nuclear ribonucleoprotein K (hnRNPK) binding ligand with Kd values of 4.6 μM and 2.6 μM measured with SPR and MST, respectively. hnRNPK-IN-1 inhibits c-myc transcription by disrupting the binding of hnRNPK and c-myc promoter. hnRNPK-IN-1 induces Hela cells apoptosis and has strongly anti-tumor activities[1].

IC50 & Target

Kd: 4.6 μM (hnRNPK; SPR assay) and 2.6 μM (hnRNPK; MST assay)[1]

In Vitro

hnRNPK-IN-1 (Compound 25; 1.25-5 μM; 24 hours) treatment induces Hela cells apoptosis could be due to its repression of cmyc transcription[1].
hnRNPK-IN-1 (1.25-5 μM; 48 hours) treatment down-regulates c-myc gene transcription and expression in Hela cells in a dose-dependent manner[1].
hnRNPK-IN-1 shows a selective anti-proliferative effect on human cancer cell lines (Siha, A549, Hela, U2OS, A375, HuH7 and HEK293 cells) with IC50 values ranged from 1.36 to 3.59 μM[1].
hnRNPK-IN-1 could selectively bind to hnRNP K without significant interaction with c-myc promoter DNA. The binding of hnRNPK-IN-1 to hnRNP K could disrupt the interaction between hnRNP K and c-myc promoter DNA in vitro and in cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]

Cell Line: Hela cells
Concentration: 1.25 μM, 2.5 μM, 5 μM
Incubation Time: 24 hours
Result: Induced Hela cells apoptosis and apoptosis-related protein expressions.

RT-PCR[1]

Cell Line: Hela cells
Concentration: 1.25 μM, 2.5 μM, 5 μM
Incubation Time: 48 hours
Result: Caused a reduction of c-myc mRNA in a dose-dependent manner in Hela cells.

Western Blot Analysis[1]

Cell Line: Hela cells
Concentration: 1.25 μM, 2.5 μM, 5 μM
Incubation Time: 48 hours
Result: The expression levels of c-MYC also decreased in a dose-dependent manner.
In Vivo

hnRNPK-IN-1 (Compound 25; 6.7-20 mg/kg; i.p.; once a day; for three weeks) exhibits good tumor growth inhibition in a Hela xenograft tumor model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c female nude mice (five weeks old) bearing Hela cells[1]
Dosage: 6.7 mg/kg, 20 mg/kg
Administration: Intraperitoneal injection; once a day; for three weeks
Result: Inhibited tumor growth in a Hela cervical xenograft.
Molecular Weight

419.43

Formula

C₂₃H₂₁N₃O₅

CAS No.
SMILES

O=C(C1=CC=C(OC)C=C1)/C=C/C2=NC3=CC=C([N+]([O-])=O)C=C3C(N4CCOCC4)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (11.92 mM; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3842 mL 11.9209 mL 23.8419 mL
5 mM 0.4768 mL 2.3842 mL 4.7684 mL
10 mM 0.2384 mL 1.1921 mL 2.3842 mL
*Please refer to the solubility information to select the appropriate solvent.
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

hnRNPK-IN-1c-MycApoptosisMychnRNPKc-myctranscriptionpromoterribonucleoproteinanticanceranti-proliferationInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
hnRNPK-IN-1
Cat. No.:
HY-135691
Quantity:
MCE Japan Authorized Agent: