2. NF-κB Immunology/Inflammation
  3. NF-κB NO Synthase
  4. Human serum albumin

Human serum albumin (HSA) is the most abundant protein in plasma and is a major determinant of plasma oncotic pressure. Human serum albumin exhibits antioxidant, anticoagulant, anti-inflammatory, anti-platelet aggregation activities as well as colloid osmotic action. Human serum albumin can block the inhibitory effect of GML on human T cells, providing protective function for T cells. Human serum albumin is also associated with cardiovascular diseases and can partially prevent the LPS (HY-D1056) induced oxidative stress, as well as the upregulation of NF-κB, NF-κB, and peroxynitrite (ONOO−) in the vascular wall, contributing to the reduction of blood pressure.
This product is recombinant Human Serum Albumin expressed in a microbial expression system.

For research use only. We do not sell to patients.

CAS No. : 70024-90-7

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Based on 4 publication(s) in Google Scholar

Human serum albumin Related Antibodies

Top Publications Citing Use of Products

    Human serum albumin purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Nov 23;42(12):113472.  [Abstract]

    The fluorescent response of mtGLP to cholesterol, triglyceride and Human serum albumin (HSA). Emission spectra of mtGLP to DOPC (40 μg/mL) in the presence of HSA (80 μg/mL), cholesterol(80μg/mL) and triglyceride (80 μg/mL) in 10.0 mM Tris-HCl buffer (pH 7.4,100.0 mMK+) with excitationatλex=405 nm.

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    nNOS iNOS eNOS

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    Description

    Human serum albumin (HSA) is the most abundant protein in plasma and is a major determinant of plasma oncotic pressure. Human serum albumin exhibits antioxidant, anticoagulant, anti-inflammatory, anti-platelet aggregation activities as well as colloid osmotic action. Human serum albumin can block the inhibitory effect of GML on human T cells, providing protective function for T cells. Human serum albumin is also associated with cardiovascular diseases and can partially prevent the LPS (HY-D1056) induced oxidative stress, as well as the upregulation of NF-κB, NF-κB, and peroxynitrite (ONOO−) in the vascular wall, contributing to the reduction of blood pressure[1][2][3].
    This product is recombinant Human Serum Albumin expressed in a microbial expression system.

    IC50 & Target

    iNOS

     

    In Vitro

    HAS (5 μM, 0-90 minutes) can bind with Glycerol Monolaurate (GML), with a Kd value of 1.4 μM. HSA can prevent the ability of GML to inhibit human T cells and protect the function of T cells[3].
    HSA (0.01, 0.05, 0.1 μM, 0-24 h) alleviates the GML-induced phosphorylation of AKT at threonine 308 and serine 473 residues as well as the formation of LAT, PLC-γ1, and AKT microclusters, and restores the production of IFN-γ, IL-2, IL-10, and TNF-α in GML-treated cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Human serum albumin Related Antibodies

    Western Blot Analysis[3]

    Cell Line: Activate peripheral blood T cells
    Concentration: 0.05 μM
    Incubation Time: 0, 2, 5, 15 min
    Result: Affected the ability of GML to inhibit AKT phosphorylation.

    RT-PCR[3]

    Cell Line: Activate peripheral blood T cells
    Concentration: 0.1, 0.05, 0.005 μM
    Incubation Time: 24h
    Result: Restored the production of IFN-γ, IL-2, IL-10, and TNF-α in GML treated cells.
    In Vivo

    Human serum albumin (10 ml/kg; Intravenous injection; Once at 1 hour and once at 5 hours; twice) partially prevents the oxidative stress induced by LPS (HY-D1056) (50 mg/kg) in male Swiss mouse models, as well as the decrease in blood pressure due to upregulation of NF-κB, iNOS, and peroxynitrite (ONOO−) in the vascular wall[4].
    Human serum albumin (10 ml/kg; Intravenous injection; Once at 1 hour and once at 5 hours; twice) in a Male Swiss mouse model completely prevented endotoxin-induced reduced vascular response to phenol catecholamines, electromyotonia, and endothelial dysfunction, and reduced inflammatory processes leading to oxidative and nitrite stress and reduced vascular reactivity[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Swiss mouse models[4]
    Dosage: 10 ml/kg; Once at 1 hour and once at 5 hours; twice
    Administration: HSA: Intravenous injection; LPS: Intraperitoneal injection (i.p.), 50 mg/kg
    Result: Prevented LPS-induced tissue NO overproduction and increased iNOS expression.
    Was effective in reducing LPS-induced oxidative stress.
    Alleviated this LPS-induced oxidative stress and inhibited the formation of peroxynitrite (ONOO−).
    Reduced LPS-induced upregulation of NF-κB.
    Clinical Trial
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    [Human serum albumin]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL

    *"≥" means soluble, but saturation unknown.

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    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.9%

    SDS (393 KB)

    COA (230 KB)

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    Human serum albumin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Human serum albumin70024-90-7HSANF-κBNO SynthaseNuclear factor-κBNuclear factor-kappaBNitric oxide synthasesNOSGlycerol monolaurateNF-KBVascularEndothelialAntioxidantAnticoagulantAnti-inflammatoryCardiovascularInhibitorinhibitorinhibit

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