IR 650 azide
IR 650 azide is a near-infrared fluorescent probe. The click chemistry reaction handle of IR 650 azide is a terminal azide, which can undergo a 'copper-catalyzed alkyne-azide cycloaddition' reaction. (Ex = 633nm, Em = 647nm)
For research use only. We do not sell to patients.
- Formula: C45H56N6O13S4
- Molecular Weight:1017.22
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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Molecular Weight 1017.22
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Formula C45H56N6O13S4
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SMILES
C[N+](C(C=C1)=C2C=C1S(=O)([O-])=O)=C(C2(CCCS(=O)(O)=O)C)/C=C/C(C3=CC=C(C=C3)CCCCC(NCCCN=[N+]=[N-])=O)=C/C=C4N(C5=C(C/4(CCCS(=O)(O)=O)C)C=C(C=C5)S(=O)(O)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
IR 650 azide Related Classifications
HY-D3253 Related Classifications
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