4-Aminobenzohydrazide
Based on 1 publication(s) in Google Scholar
4-Aminobenzohydrazide (p-Aminobenzohydrazide) is an irreversible myeloperoxidase (MPO) inhibitor (IC50=0.3 μM) that induces oxidative stress burst in neutrophils (ROS IC50=43.6 μM). 4-Aminobenzohydrazide has been used in subacute stroke research.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.21%
- CAS 番号: 5351-17-7
- 分子式: C7H9N3O
- 分子量:151.17
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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MedChemExpress（MCE）の使用を引用している文献 4-Aminobenzohydrazide
More
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Neutrophil | IC50 |
30.6 μM
Compound: ABAH
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Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay
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[PMID: 23871908] |
| Neutrophil | IC50 |
43.6 μM
Compound: ABAH
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Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay
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[PMID: 23871908] |
体外実験
4-Aminobenzohydrazide reduces PMA-induced APF-dependent fluorescence signals (HO· and ONOO- levels) in human neutrophils with an IC50 of 43.6 μM[3].
4-Aminobenzohydrazide inhibits the oxidative burst in PMA-stimulated human neutrophils as assessed by fluorescence assay, with an IC50 of 30.6 μM for HOCl-induced APF oxidation inhibition after 6 minutes[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 5351-17-7
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性状 Solid
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分子量 151.17
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分子式 C7H9N3O
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Color White to off-white
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SMILES
O=C(NN)C1=CC=C(N)C=C1
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別名
p-Aminobenzohydrazide; p-Aminobenzoic acid hydrazide
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Dev Cell
A single fluorescent probe to examine the dynamics of mitochondria-lysosome interplay and extracellular vesicle role in ferroptosis. [Abstract]2024 Feb 26;59(4):517-528.e3. PMID: 38272028
溶剤 & 溶解度
体外:
DMSO : ≥ 25 mg/mL (165.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (6.62 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (6.62 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
純度とドキュメンテーション
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データシート (278 KB)
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SDS (393 KB)
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- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Kettle AJ, et al. Inhibition of myeloperoxidase by benzoic acid hydrazides. Biochem J. 1995 Jun 1;308 ( Pt 2)(Pt 2):559-63. [Content Brief]
[2]. Forghani R, et al. Myeloperoxidase propagates damage and is a potential therapeutic target for subacute stroke. J Cereb Blood Flow Metab. 2015 Mar;35(3):485-93. [Content Brief]
[3]. Ribeiro D, et al. Modulation of human neutrophils' oxidative burst by flavonoids. Eur J Med Chem. 2013 Sep;67:280-92. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.6151 mL | 33.0753 mL | 66.1507 mL | 165.3767 mL |
| 5 mM | 1.3230 mL | 6.6151 mL | 13.2301 mL | 33.0753 mL | |
| 10 mM | 0.6615 mL | 3.3075 mL | 6.6151 mL | 16.5377 mL | |
| 15 mM | 0.4410 mL | 2.2050 mL | 4.4100 mL | 11.0251 mL | |
| 20 mM | 0.3308 mL | 1.6538 mL | 3.3075 mL | 8.2688 mL | |
| 25 mM | 0.2646 mL | 1.3230 mL | 2.6460 mL | 6.6151 mL | |
| 30 mM | 0.2205 mL | 1.1025 mL | 2.2050 mL | 5.5126 mL | |
| 40 mM | 0.1654 mL | 0.8269 mL | 1.6538 mL | 4.1344 mL | |
| 50 mM | 0.1323 mL | 0.6615 mL | 1.3230 mL | 3.3075 mL | |
| 60 mM | 0.1103 mL | 0.5513 mL | 1.1025 mL | 2.7563 mL | |
| 80 mM | 0.0827 mL | 0.4134 mL | 0.8269 mL | 2.0672 mL | |
| 100 mM | 0.0662 mL | 0.3308 mL | 0.6615 mL | 1.6538 mL |
4-Aminobenzohydrazide 関連分類
HY-B0880 関連分類
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