Cy 3 (Non-Sulfonated)
Cy 3 Non-Sulfonated (Cyanine3) is a cyanine (Cy) dye, and a fluorescent label with green channel for protein and nucleic acid. Cy 3 Non-Sulfonated is a fluorescent photoproduct of Cyanine5 via photoconversion upon photoexcitation. Cy 3 Non-Sulfonated can be used to high-density single-particle tracking in a living cell without using UV illumination and cell-toxic additives (Ex=470 nm; Em=515 nm and 565 nm nm).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 146397-20-8
- 分子式: C43H50N4O14S2
- 分子量:911.01
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
Labeling of Protein:
1. Add protein (100 μL) to Cy 3 Non-Sulfonated in 96 black micro-well for 50 min at 37 °C. Incubate the cells according to your normal protocol.
2. Scan plate using BioTek and collect data with excitation wavelength of 470 nm, and emission wavelength of 515 nm and 565 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
570
化学情報
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CAS 番号 146397-20-8
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分子量 911.01
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分子式 C43H50N4O14S2
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SMILES
O=S(C1=CC2=C([N+](CCCCCC(ON3C(CCC3=O)=O)=O)=C(/C=C/C=C4N(CCCCCC(ON5C(CCC5=O)=O)=O)C6=C(C=C(S(=O)(O)=O)C=C6)C/4(C)C)C2(C)C)C=C1)([O-])=O
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別名
Cyanine3
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Cho Y, et al. Mechanism of Cyanine5 to Cyanine3 Photoconversion and Its Application for High-Density Single-Particle Tracking in a Living Cell. J Am Chem Soc. 2021 Sep 8;143(35):14125-14135. [Content Brief]
[2]. Li H, et al. Silver enhanced ratiometric nanosensor based on two adjustable Fluorescence Resonance Energy Transfer modes for quantitative protein sensing. Biosens Bioelectron. 2017 Jan 15;87:428-432. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Cy 3 (Non-Sulfonated) 関連分類
HY-D0968 関連分類
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