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製品番号: HY-186091

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AcdK

AcdK is a non-natural amino acid and a precursor of allysine. AcdK allows site-specific incorporation into target proteins in E. coli via the amber suppression strategy. AcdK enables site-specific lysine dimethylation or monomethylation modification of target proteins. AcdK can synthesize site-specific lysine-methylated variants of histone H3 and p53, which is applicable for investigating the substrate specificity and catalytic function of epigenetic enzymes.

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CAS 番号: 2097535-26-5
分子式: C₁₄H₁₇N₅O₄
分子量:319.32

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (263 KB)

AcdK 構造式

CAS 番号: 2097535-26-5

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

AcdK (0.5 mM) is efficiently and site-specifically incorporated into sfGFP at position D134 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable quantitative conversion to sfGFP with site-specific lysine dimethylation at position D134^[1].
AcdK (2 mM) is efficiently and site-specifically incorporated into histone H3 at positions K4, K9, or K36 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable conversion to site-specific dimethylated histone H3 variants^[1].
AcdK is efficiently and site-specifically incorporated into p53 at position K372 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable conversion to p53 with site-specific lysine mono- or dimethylation at K372, which directly enhances Tip60-catalyzed acetylation of p53 at K120 by 2.5- to 3-fold^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

化学情報

CAS 番号 2097535-26-5
分子量 319.32
分子式 C₁₄H₁₇N₅O₄
SMILES

OC([C@@H](N)CC/C=C/NC(OCC1=CC=C(C=C1)N=[N+]=[N-])=O)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (263 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Wang ZA, et al. A Genetically Encoded Allysine for the Synthesis of Proteins with Site-Specific Lysine Dimethylation. Angew Chem Int Ed Engl. 2017;56(1):212-216. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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分子量分子量

希釈計算機

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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