country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

製品番号: HY-122081

Copy Link Print Information

CPI-644

CPI-644 is a selective EP300/CBP bromodomain inhibitor, with an IC₅₀ of 0.18 µM against the CBP bromodomain. CPI-644 reduces the proportion of FOXP3⁺cells and downregulates the expression of LAG-3, CTLA-4 and PD-1. CPI-644 can be used in cancer-related research.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 1904647-34-2
分子式: C₁₈H₁₉N₃O₃S
分子量:357.43

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

CPI-644 構造式

CAS 番号: 1904647-34-2

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

Histone Acetyltransferase アイソフォーム固有の製品をすべて表示 :

体外実験

CPI-644 potently and selectively inhibits the purified CBP bromodomain in AlphaLISA peptide competition assays, with an IC₅₀ of 0.18 μM^[1].
CPI-644 binds tightly to the purified CBP bromodomain, with a K_d value of 0.084 μM measured by ITC^[1].
CPI-644 inhibits the cellular interaction between the bromodomains of CBP and EP300 and histone H3.3 in HEK293 cells, with EC₅₀ values of 0.53 μM and 0.36 μM, respectively^[1].
CPI-644 potently inhibits the differentiation of naive human CD4⁺CD45RA⁺ T cells into FOXP3⁺ Treg, with an IC₅₀ of 1.8 μM^[1].
CPI-644 dose-dependently reduces the expression of Treg inhibitory markers LAG-3, CTLA-4 and PD-1 in in vitro differentiated regulatory T cells (Treg), with EC₅₀ values of 0.9 μM, 1.8 μM and 4.3 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

化学情報

CAS 番号 1904647-34-2
分子量 357.43
分子式 C₁₈H₁₉N₃O₃S
SMILES

C(=C/C1=C(S(N)(=O)=O)C=CC=C1)\C2=C3C(NC(=O)C[C@@H](C)N3)=CC=C2
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

プロトコル

All Molecular Biology Cell Biology

RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Ghosh S, et al. Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition. J Biol Chem. 2016;291(25):13014-13027. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

お問い合わせ在庫あり

Other size

お問い合わせ