Digoxigenin NHS ester
Based on 1 Customer Validation
Digoxigenin NHS ester is an activated ester which readily reacts with amino groups under mild conditions, attaching the Digoxigenin (HY-B1025) moiety to proteins or amino-. Digoxigenin NHS ester can be used to label proteins and oligonucleotides.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.00%
- CAS 番号: 129273-26-3
- 分子式: C35H50N2O10
- 分子量:658.78
-
保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
生物活性
Digoxigenin NHS ester (1 mg/mL, overnight) after reacting with R-phycocyanin solution can serve as the donor in the fluorescence energy transfer (FRET) system, enabling highly sensitive detection of Digoxin (HY-B1049)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 129273-26-3
-
性状 Solid
-
分子量 658.78
-
分子式 C35H50N2O10
-
Color White to off-white
-
SMILES
O[C@@]12[C@@]([C@@H](C(CO3)=CC3=O)CC1)([C@@H](C[C@@]4([H])[C@@]2([H])CC[C@@]5([H])[C@@]4(CC[C@@H](C5)OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C)O)C
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 100 mg/mL (151.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (271 KB)
-
SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
-
取扱説明書 (2659 KB)
参考文献
[2]. Dalia BURZYN, et al. Extracellular vesicle-aso constructs targeting stat6. Patent, WO2021030776A1.
[3]. Graefe KA, et al. High-performance liquid chromatography with on-line post-column immunoreaction detection of digoxin and its metabolites based on fluorescence energy transfer in the far-red spectral region. J Chromatogr B Biomed Sci Appl. 2000 Aug 18;745(2):305-14. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5180 mL | 7.5898 mL | 15.1796 mL | 37.9489 mL |
| 5 mM | 0.3036 mL | 1.5180 mL | 3.0359 mL | 7.5898 mL | |
| 10 mM | 0.1518 mL | 0.7590 mL | 1.5180 mL | 3.7949 mL | |
| 15 mM | 0.1012 mL | 0.5060 mL | 1.0120 mL | 2.5299 mL | |
| 20 mM | 0.0759 mL | 0.3795 mL | 0.7590 mL | 1.8974 mL | |
| 25 mM | 0.0607 mL | 0.3036 mL | 0.6072 mL | 1.5180 mL | |
| 30 mM | 0.0506 mL | 0.2530 mL | 0.5060 mL | 1.2650 mL | |
| 40 mM | 0.0379 mL | 0.1897 mL | 0.3795 mL | 0.9487 mL | |
| 50 mM | 0.0304 mL | 0.1518 mL | 0.3036 mL | 0.7590 mL | |
| 60 mM | 0.0253 mL | 0.1265 mL | 0.2530 mL | 0.6325 mL | |
| 80 mM | 0.0190 mL | 0.0949 mL | 0.1897 mL | 0.4744 mL | |
| 100 mM | 0.0152 mL | 0.0759 mL | 0.1518 mL | 0.3795 mL |