Flubida-2

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. Dissolve Flubida-2 in DMSO (2 mM).
2. Mix the stock solution 1: 1 with 20% (w/v in DMSO) Pluronic F-127, and dilute to the desired final concentration (2-4 μM) with serum-free DMEM.
3. 30 to 48 h post-transfection with either AV-KDEL or ST-AV DNA, cells are rinsed once with serum-free DMEM and loaded with 2-4 μM Flubida-2 for 3-5 h (or overnight for 10-15 h).
4. Chase the labeled cells with normal growth medium for at least 2 h, to allow excess dye-biotin to exit from the cytosol.
5. The strong avidin-biotin interaction ensures stable, specific avidin-Flubi- binding that resisted washing. Biotin starvation of the cells is not necessary before Flubida- loading, as staining was bright and stable.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.