Fusarochromanone
Fusarochromanone (FC-101) is a fungal metabolite with potent anti-angiogenic and anti-cancer activity. Fusarochromanone-activated JNK pathway is attributed to induction of reactive oxygen species (ROS).
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- CAS 番号: 802915-53-3
- 分子式: C15H20N2O4
- 分子量:292.33
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
Fusarochromanone (FC101; 10 μM; 24 hours) induces apoptosis and an increase in proportion of cells in the sub-G1 phase in both HaCat and P9-WT cell lines[1].
Fusarochromanone (FC101; 0-1 μM; 24 h) induces the cleavage of both caspase-3 and PARP, a well-known substrate for activated caspases. FC101 does not affect the expression of the anti-apoptotic proteins, Bcl-2, Bcl-XL, Mcl-1, or the pro-apoptotic proteins BAD, BAK, BAX[1].
Fusarochromanone (FC101) exhibits very potent in-vitro growth inhibitory effects (IC50 ranging from 10 nM-2.5 μM) against HaCat (pre-malignant skin), P9-WT (malignant skin), MCF-7 (low malignant breast), MDA-231 (malignant breast), SV-HUC (premalignant bladder), UM-UC14 (malignant bladder), and PC3 (malignant prostate) in a time-course and dose-dependent manner, with the UM-UC14 cells being the most sensitive.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HaCat and P9-WT cell lines
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Concentration:10 μM
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Incubation Time:24 hours
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Result:Showed cells in the G2 and M phases of the cell cycle for both cell lines.
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Cell Line:MDA-MB-231 cells
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Concentration:0.05 μM, 0.1 μM, 0.2 μM, 0.5 μM, 1 μM
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Incubation Time:24 hours
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Result:Induced the cleavage of both caspase-3 and PARP.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SCID Beige mice (CB17/Icr.Cg-PrkdcscidLystbg/Crl) injected with SRB12-p9 cells[1]
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Dosage:8 mg/kg
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Administration:IP; 5 days per week; for 3.5 weeks
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Result:Achieved a 30% reduction in tumor size at a dose of 8 mg/kg/day.
化学情報
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CAS 番号 802915-53-3
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分子量 292.33
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分子式 C15H20N2O4
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SMILES
O=C1CC(C)(C)OC2=CC=C(C(C[C@@H](N)CO)=O)C(N)=C12
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別名
FC-101
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
純度とドキュメンテーション
参考文献
[1]. Elahe Mahdavian, et al.Biological activities of fusarochromanone: a potent anti-cancer agent. BMC Res Notes. 2014 Sep 3;7:601. [Content Brief]
[2]. Ying Gu, et al.Fusarochromanone-induced reactive oxygen species results in activation of JNK cascade and cell death by inhibiting protein phosphatases 2A and 5. Oncotarget. 2015 Dec 8;6(39):42322-33. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Fusarochromanone 関連分類
HY-136901 関連分類
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